Cruz T F, Quackenbush E J, Letarte M, Moscarello M A
Neurochem Res. 1986 Jun;11(6):877-89. doi: 10.1007/BF00965211.
The levels of a glycoprotein reactive with monoclonal antibody (MAb) 44D10 in white and gray matter from brains of victims of several neurological diseases, including Multiple Sclerosis, Alzheimer's, Parkinson's and Huntington's diseases, were compared to that of normal individuals. The concentration of antigen reactive with MAb 44D10 was elevated in both gray and white matter of all MS brains examined, but not in brains with other neurological diseases. The increase in the concentration of antigen varied amongst the MS brains, such that the levels of antigen were only slightly increased in 2 of the 6 MS brains whereas 2 to 4 fold higher levels were found in the other 4 brains. Increased levels of antigen were detected in gray matter of MS brains, whereas this antigen was either not detected or present in very low levels in gray matter homogenates prepared from age-matched normal brains. MAb Leu 1, which reacts with T lymphocytes, was not absorbed by normal and MS brain tissue suggesting the increase in antigen reactive with MAb 44D10 in MS brain homogenates was not associated with non-specific infiltration by T lymphocytes. Comparison of the purified antigen from MS gray matter and normal white matter by gel electrophoresis demonstrated that MAb 44D10 was reacting with a similar protein in both tissues with an apparent molecular weight of 80K. We have named this molecule P-80 glycoprotein.
将患有包括多发性硬化症、阿尔茨海默病、帕金森病和亨廷顿病在内的几种神经疾病患者大脑白质和灰质中与单克隆抗体（MAb）44D10反应的糖蛋白水平，与正常个体的进行了比较。在所有检测的多发性硬化症患者大脑的灰质和白质中，与MAb 44D10反应的抗原浓度均升高，但在患有其他神经疾病的大脑中未升高。抗原浓度的增加在多发性硬化症患者大脑之间有所不同，例如在6例多发性硬化症患者大脑中的2例中，抗原水平仅略有升高，而在其他4例大脑中则发现其水平高出2至4倍。在多发性硬化症患者大脑的灰质中检测到抗原水平升高，而在从年龄匹配的正常大脑制备的灰质匀浆中，要么未检测到该抗原，要么其含量极低。与T淋巴细胞反应的MAb Leu 1未被正常和多发性硬化症患者脑组织吸收，这表明多发性硬化症患者脑匀浆中与MAb 44D10反应的抗原增加与T淋巴细胞的非特异性浸润无关。通过凝胶电泳对来自多发性硬化症患者灰质和正常白质的纯化抗原进行比较，结果表明MAb 44D10与两种组织中一种表观分子量为80K的相似蛋白质发生反应。我们将该分子命名为P - 80糖蛋白。
