Eunice Kennedy Shriver National Institute of Child Health and Human Development, Reproductive Endocrinology and Infertility Fellowship Program, National Institutes of Health, Bethesda, Maryland.
Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Sidney Kimmel Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania.
Fertil Steril. 2018 Mar;109(3):460-466.e2. doi: 10.1016/j.fertnstert.2017.11.002. Epub 2018 Feb 7.
To compare the transcriptome of cumulus cells associated with a euploid embryo that resulted in live birth with that of a sibling euploid embryo without sustained implantation.
Paired analysis.
Academic institution.
PATIENT(S): Couples undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection with preimplantation genetic screening with female age ≤42 years and normal ovarian reserve.
INTERVENTION(S): Transcriptome profiling of cumulus cells from sibling oocytes for correlation with live birth after euploid blastocyst transfer. Embryos were individually cultured to facilitate association with clinical outcomes. The cumulus cell transcriptome from the embryo resulting in live birth was compared with that of its sibling embryo without sustained implantation to investigate potential biomarkers that may aid in embryo selection.
MAIN OUTCOME MEASURE(S): Differential gene expression in cumulus cells associated with a euploid embryo resulting in live birth and its sibling euploid embryo without sustained implantation using next-generation RNA sequencing (RNAseq).
RESULT(S): Cumulus cell RNAseq of 34 samples (from 17 patients) generated an average of 10.4 ± 4 × 10 reads per sample. A total of 132 differentially expressed genes between sibling embryos that resulted in a live birth and those that did not were identified (P<.05). However, after correcting for multiple testing none of the genes remained significantly differentially expressed (false discovery rate <.05).
CONCLUSION(S): The RNAseq profiles were similar between cumulus cells associated with a euploid embryo resulting in live birth and its sibling embryo that did not sustain implantation. The cumulus cell transcriptome is not predictive of live birth within an individual patient's cohort of euploid embryos.
比较与活产胚胎相关的正常二倍体卵裂期胚胎的卵丘细胞转录组与未持续着床的同卵正常二倍体胚胎的转录组。
配对分析。
学术机构。
接受体外受精（IVF）/卵胞浆内单精子注射（ICSI）联合胚胎植入前遗传学筛查的夫妇，女性年龄≤42 岁，卵巢储备正常。
对同卵卵母细胞的卵丘细胞进行转录组谱分析，以关联正常二倍体囊胚移植后的活产。胚胎单独培养以利于与临床结局相关联。将导致活产的胚胎的卵丘细胞转录组与未持续着床的同卵正常二倍体胚胎进行比较，以研究可能有助于胚胎选择的潜在生物标志物。
使用下一代 RNA 测序（RNAseq）比较与正常二倍体胚胎导致活产相关的卵丘细胞与未持续着床的同卵正常二倍体胚胎的差异基因表达。
34 个样本（来自 17 名患者）的卵丘细胞 RNAseq 平均每个样本产生 10.4±4×10 个读数。在导致活产和未活产的同卵胚胎之间共鉴定出 132 个差异表达基因（P<.05）。然而，经过多重检验校正后，没有一个基因的表达差异具有统计学意义（错误发现率<.05）。
与活产正常二倍体胚胎相关的卵丘细胞的 RNAseq 图谱与未持续着床的同卵胚胎相似。卵丘细胞转录组不能预测个体患者队列中正常二倍体胚胎的活产。
