Abouhajer Fathi, El-Ashram Saeed, Karama Musafiri, Huang Shujian, Liu Jian-Feng
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Faculty of Education, Asmarya University for Islamic Sciences, Zliten, Libya.
Funct Integr Genomics. 2018 May;18(3):277-285. doi: 10.1007/s10142-018-0589-9. Epub 2018 Feb 10.
We have set up an ex vivo ovine ruminal model, which can mimic the multicellular process to explore the early steps in Salmonella typhimurium lipopolysaccharide (LPS) stimulation using RNA-seq technology. Ovine ruminal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. A total of 8 and 523 genes were significantly over-expressed between LPS-treated and control tissues at 6 and 12 h, respectively. However, six and seven hundred and thirteen genes were substantially repressed between the aforementioned tissues, correspondingly. Key genes up-regulated in response to the addition of LPS were tumor necrosis factor (TNF), interlukin (IL)-1 beta(b), IL-6, IL-8, IL-17B, IL-19, MMP-1, MMP-3, and integrin alpha 2 (ITGA8, 9). This study shows for the first time that galectin-1 is up-regulated in an ex vivo ruminal segment model exposed to bacterial lipopolysaccharide following 6 h of incubation. The ruminal segment model has been shown to be a suitable tool to study the bacterial lipopolysaccharide effects on the ovine ruminal tissues prior to in vivo assessment.
我们建立了一种体外绵羊瘤胃模型，该模型可以模拟多细胞过程，利用RNA测序技术探索鼠伤寒沙门氏菌脂多糖（LPS）刺激的早期步骤。收集绵羊瘤胃外植体进行组织学和转录分析，并收集上清液以定量乳酸脱氢酶（LDH）。在6小时和12小时时，LPS处理组和对照组组织之间分别有8个和523个基因显著过度表达。然而，上述组织之间相应地分别有6个和713个基因被显著抑制。添加LPS后上调的关键基因有肿瘤坏死因子（TNF）、白细胞介素（IL）-1β、IL-6、IL-8、IL-17B、IL-19、基质金属蛋白酶-1（MMP-1）、MMP-3和整合素α2（ITGA8、9）。本研究首次表明，在体外瘤胃段模型中，经6小时孵育后，暴露于细菌脂多糖时半乳糖凝集素-1会上调。瘤胃段模型已被证明是在体内评估之前研究细菌脂多糖对绵羊瘤胃组织影响的合适工具。
