Day Christopher J, Korolik Victoria
Institute for Glycomics, Griffith University, Southport, QLD, Australia.
Methods Mol Biol. 2018;1729:303-317. doi: 10.1007/978-1-4939-7577-8_24.
Ligand-receptor interactions triggering signal transduction components of many sensory pathways, remain elusive due to paucity of high-throughput screening methods. Here we describe our use of small molecule microarrays comprising of small glycans, amino and organic acids, salts, and other known chemoeffectors, for screening of ligands specific to various sensory receptors, followed by surface plasmon resonance to verify the veracity and to determine the affinity constants of the interactions. This methodology allows for rapid and identification of the direct ligand binding between the sensory receptors and their specific ligands.
由于缺乏高通量筛选方法，触发许多感觉通路信号转导成分的配体 - 受体相互作用仍不明确。在此，我们描述了我们使用由小聚糖、氨基酸、有机酸、盐和其他已知化学效应物组成的小分子微阵列，用于筛选各种感觉受体特异性的配体，随后进行表面等离子体共振以验证相互作用的准确性并确定其亲和常数。这种方法能够快速鉴定感觉受体与其特定配体之间的直接配体结合。
