Marie J P, Vernant J P, Dreyfus B, Breton-Gorius J
Br J Haematol. 1979 Dec;43(4):549-58. doi: 10.1111/j.1365-2141.1979.tb03787.x.
Twelve cases of Philadelphia chromosome positive chronic granulocytic leukaemia (CGL) in blast transformation have been investigated using ultrastructural peroxidase detection. In all cases, the leukaemic blasts were negative for myeloperoxidase on the basis of standard cytochemistry. In nine cases a variable proportion of blasts contained peroxidase activity detectable only by electron microscopy, permitting definition of their myeloid nature. By their distinct characteristics and localization, different peroxidase activities were recognized. Thus, several types of blasts were identified: megakaryoblasts (MKB), basophil promyelocytes (BPM), myeloid blasts with small granules containing peroxidase (MyB), and proerythroblasts (ProE). MKB were predominant in two cases and present in four cases, mixed with other myeloid blasts. BPM were abundant in one case and present in seven cases. MyB were identified as a majority in four cases. Three cases remained without any peroxidase. It is concluded that ultrastructural detection of peroxidases is of value for the identification of early myeloid blasts. Their high incidence and the simultaneous presence of several myeloid precursors suggest that during the blast crisis the target cell is frequently a pluripotent myeloid stem cell.
运用超微结构过氧化物酶检测法对12例费城染色体阳性的慢性粒细胞白血病（CGL）急变期病例进行了研究。在所有病例中，根据标准细胞化学方法，白血病原始细胞髓过氧化物酶呈阴性。9例中，一定比例的原始细胞含有仅通过电子显微镜才能检测到的过氧化物酶活性，从而确定了它们的髓系性质。根据其独特的特征和定位，识别出了不同的过氧化物酶活性。因此，确定了几种类型的原始细胞：巨核母细胞（MKB）、嗜碱性早幼粒细胞（BPM）、含有过氧化物酶小颗粒的髓系原始细胞（MyB）和原红细胞（ProE）。MKB在2例中占主导地位，4例中存在，与其他髓系原始细胞混合。BPM在1例中大量存在，7例中存在。MyB在4例中占多数。3例未检测到任何过氧化物酶。结论是，过氧化物酶的超微结构检测对于早期髓系原始细胞的识别具有价值。它们的高发生率以及几种髓系前体细胞的同时存在表明，在急变期，靶细胞通常是多能髓系干细胞。
