Qiao Jing, Ren Guang-xi, Li Yan-peng, Zhang Quan, Qi Meng-die, Liu Yong, Liu Chun-sheng
Zhong Yao Cai. 2016 May;39(5):956-61.
To study the effect of chemical constituents from Glycyrrhiza uralensis induced by exogenous 6-benzylaminopurine( 6-BA) in different growth periods, aim to explore the regulatory network of Glycyrrhiza uralensis.
The transplants of two-year-old Glycyrrhiza uralensis were subjected to four concentrations of 6-BA( 15,20,50,100 mg / L) at June and July. The content of seven kinds of chemical constituents including glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, liquiritin apioside,isoliquiritin apioside were determined by HPLC. Then long-term dynamic changes of the content of seven chemical compositions were analyzed.
In June, the content of seven compositions increased remarkably after 6-BA stimulating than that in July. Seven kinds of chemical constituents increased remarkably after 100 mg / L 6-BA stimulating for 3 months, and the increase rates were59. 34%,71. 14%,57. 31%,16. 36%,30. 17%,80. 26%,91. 90%,respectively. The greatest impact was glycyrrhizic acid with different concentration stimulating. 6-BA stress had a protection ability against the decrease of flavonoids in licorice under natural growth condition. The treatment of 6-BA had effect to the content of Glycyrrhiza uralensis,but hardly altered the ratio of their chemical composition.
A regulatory network is existed among medicinal ingredients of Glycyrrhiza uralensis. Glycyrrhizic acid and flavonoids accumulation of Glycyrrhiza uralensis can be affected by exogenous 6-BA stimulation in certain case.
研究外源6-苄氨基嘌呤（6-BA）对不同生长时期乌拉尔甘草化学成分的影响，旨在探索乌拉尔甘草的调控网络。
在6月和7月，对两年生乌拉尔甘草移栽苗施加4种浓度（15、20、50、100mg/L）的6-BA。采用高效液相色谱法测定甘草酸、甘草苷、异甘草苷、甘草素、异甘草素、甘草苷元芹糖甘草苷、异甘草苷元芹糖甘草苷7种化学成分的含量。然后分析7种化学成分含量的长期动态变化。
6月，6-BA刺激后7种成分的含量比7月显著增加。100mg/L 6-BA刺激3个月后，7种化学成分显著增加，增加率分别为59.34%、71.14%、57.31%、16.36%、30.17%、80.26%、91.90%。不同浓度刺激下对甘草酸的影响最大。6-BA胁迫对自然生长条件下甘草黄酮类化合物含量的降低具有保护作用。6-BA处理对乌拉尔甘草的含量有影响，但对其化学成分比例影响不大。
乌拉尔甘草药用成分之间存在调控网络。在一定情况下，外源6-BA刺激可影响乌拉尔甘草甘草酸和黄酮类化合物的积累。
