Jarrell B E, Williams S K, Stokes G, Hubbard F A, Carabasi R A, Koolpe E, Greener D, Pratt K, Moritz M J, Radomski J
Surgery. 1986 Aug;100(2):392-9.
Endothelial seeding of vascular graft surfaces may lead to a less thrombogenic surface. We examined the feasibility of using microvessel endothelial cells derived from human fat for seeding purposes. Human fat was treated with collagenase for 24 minutes, washed, and purified in a Percoll gradient separation. This yielded 1.25 +/- 0.45 X 10(6) cells/gm of fat. After a 1-hour incubation on plasma-coated Dacron, 2.8 +/- 1.5 X 10(4) cells remained firmly adherent to the surface. When exposed to flow for 2 hours at a shear stress of 0 to 80 dyne/cm2, between 50% and 100% of the initially adherent cells remained adherent. Statistical analysis of this data failed to demonstrate a strong relationship between the number of adherent cells and the shear rate. Scanning electron microscopy demonstrated endothelial cells in various stages of attachment to the plasma-coated Dacron. Although most cells were still round and only focally attached to the surface, some cells were maximally flattened, forming cell-to-cell contact. Because of the high cell yield and the firm adherence characteristics, we conclude that microvessel endothelial cells may offer the possibility for confluent endothelial cell seeding of a graft at the time of surgical implantation without the need for cell culture.
在血管移植物表面进行内皮细胞接种可能会产生血栓形成倾向较低的表面。我们研究了使用源自人脂肪的微血管内皮细胞进行接种的可行性。将人脂肪用胶原酶处理24分钟，洗涤后，在Percoll梯度分离中进行纯化。每克脂肪可获得1.25±0.45×10⁶个细胞。在血浆包被的涤纶上孵育1小时后，有2.8±1.5×10⁴个细胞牢固地黏附于表面。当在0至80达因/平方厘米的剪切应力下暴露于流动环境2小时后，最初黏附的细胞中有50%至100%仍保持黏附状态。对该数据的统计分析未能证明黏附细胞数量与剪切速率之间存在强相关性。扫描电子显微镜显示内皮细胞处于黏附于血浆包被涤纶的不同阶段。尽管大多数细胞仍呈圆形，仅局部黏附于表面，但一些细胞已最大限度地扁平，形成细胞间接触。由于细胞产量高且具有牢固的黏附特性，我们得出结论，微血管内皮细胞可能为手术植入时在移植物上进行融合内皮细胞接种提供可能性，而无需细胞培养。
