心肌梗死患者的血浆CXCL1水平及TRAF3IP2基因变异
Plasma CXCL1 levels and TRAF3IP2 variants in patients with myocardial infarction.
作者信息
Pordel Safoora, Sajedi Khanian Mahdi, Karimi Mohammad Hossein, Nikoo Hossein, Doroudchi Mehrnoosh
机构信息
Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Cardiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
J Clin Lab Anal. 2018 Jul;32(6):e22402. doi: 10.1002/jcla.22402. Epub 2018 Feb 12.
BACKGROUND
IL-17A plays an important role in inflammatory responses in myocardial infarction (MI). IL-17A signals through its receptor, for which, Act1 (TRAF3IP2) functions as a key upstream adaptor in the pathway.
AIM
To compare frequencies of functional polymorphisms of TRAF3IP2 (rs13210247, rs33980500) between patients with MI and healthy controls.
METHODS
The selected SNPs were studied in 201 Iranian MI patients and 201 healthy blood donors from Fars Province by PCR-RFLP in association with clinicopathologic criteria of patients. CXCL1 plasma levels in 126 MI patients and 50 normal subjects were measured by ELISA.
RESULTS
A significant increase in the mutant (T) allele of TRAF3IP2 rs33980500 in patients with diastolic dysfunction of the heart (P = .01) was observed. The highest correlation, however, was observed between the TRAF3IP2 rs33980500 TT genotype and T allele with left main coronary artery stenosis (P = .01, P < .001; OR = 31.03). T allele of TRAF3IP2 rs33980500 was also associated with female gender, family history of cardiovascular disease, and mechanical complications of heart (P = .04, P = .02, and P = .01, respectively). Moreover, TRAF3IP2 rs13210247 (G) correlated with mechanical complications of the heart (P = .01). A significant increase in the plasma levels of CXCL1 chemokine in patients (P = .0006) associated with TT genotype of TRAF3IP2 (rs33980500) was observed (P = .04).
CONCLUSION
The gene variants of Act1 adaptor are associated with correlates of poor outcome in patients with MI and plasma CXCL1 levels.
背景
白细胞介素-17A（IL-17A）在心肌梗死（MI）的炎症反应中起重要作用。IL-17A通过其受体发出信号，其中，Act1（TRAF3IP2）在该信号通路中作为关键的上游衔接蛋白发挥作用。
目的
比较MI患者与健康对照者中TRAF3IP2（rs13210247、rs33980500）功能多态性的频率。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，结合患者的临床病理标准，对201例来自法尔斯省的伊朗MI患者和201例健康献血者的选定单核苷酸多态性（SNP）进行研究。采用酶联免疫吸附测定（ELISA）法检测126例MI患者和50例正常受试者血浆中CXC趋化因子配体1（CXCL1）水平。
结果
观察到心脏舒张功能障碍患者中TRAF3IP2 rs33980500的突变（T）等位基因显著增加（P = 0.01）。然而，观察到TRAF³IP2 rs33980500的TT基因型和T等位基因与左主干冠状动脉狭窄之间的相关性最高（P = 0.01，P < 0.001；比值比[OR]=31.03）。TRAF³IP2 rs33980500的T等位基因还与女性性别、心血管疾病家族史以及心脏机械并发症相关（分别为P = 0.04、P = 0.02和P = 0.01）。此外，TRAF³IP2 rs13210247（G）与心脏机械并发症相关（P = 0.01）。观察到与TRAF³IP2（rs33980500）的TT基因型相关的患者血浆中CXCL1趋化因子水平显著升高（P = 0.0006）（P = 0.04）。
结论
Act1衔接蛋白的基因变异与MI患者不良预后的相关因素以及血浆CXCL1水平相关。
Zotero 插件