Petersen J
Ann Rheum Dis. 1986 Jul;45(7):539-45. doi: 10.1136/ard.45.7.539.
A reverse haemolytic plaque forming cell (PFC) assay was employed to analyse the impact of T suppressor/cytotoxic and T helper cells on B cell function in 10 patients with rheumatoid arthritis (RA). In all cases T8-enriched cells from synovial fluid and blood suppressed the pokeweed mitogen (PWM) induced IgM, IgG, and IgA secretion by autologous lymphocytes to the same degree. The suppression was partly abolished by irradiation of T8-enriched cells. T4-enriched cells from blood increased the PWM induced Ig secretion by autologous blood B cells. In six of 10 patients responses 1.2 to four times higher were obtained with T4-enriched cells from synovial fluid, but in four of 10 patients synovial fluid T4-enriched cells did not increase the PWM responses of blood B cells. T4- and T8-enriched T cells from synovial fluid comprised more Ia+ cells than did T cells from blood (36% v 3% and 43% v 6%). Ia+ T helper and suppressor/cytotoxic cells may modulate in vivo activation of synovial B cells in RA.
采用反向溶血空斑形成细胞(PFC)试验分析10例类风湿关节炎(RA)患者中抑制性/细胞毒性T细胞和辅助性T细胞对B细胞功能的影响。在所有病例中,来自滑液和血液的富含T8细胞抑制了美洲商陆有丝分裂原(PWM)诱导的自体淋巴细胞分泌IgM、IgG和IgA,抑制程度相同。对富含T8细胞进行照射可部分消除这种抑制作用。来自血液的富含T4细胞增加了PWM诱导的自体血液B细胞分泌Ig。10例患者中有6例,来自滑液的富含T4细胞使反应提高了1.2至4倍,但10例患者中有4例,滑液中富含T4细胞并未增加血液B细胞的PWM反应。与来自血液的T细胞相比,来自滑液的富含T4和T8的T细胞包含更多Ia +细胞(分别为36%对3%和43%对6%)。Ia +辅助性T细胞和抑制性/细胞毒性T细胞可能在体内调节RA患者滑膜B细胞的激活。