Petersen J, Heilmann C, Høier-Madsen M
Rheumatol Int. 1986;6(3):115-20. doi: 10.1007/BF00270347.
Lymphocytes from patients with rheumatoid arthritis (RA) were assayed for their ability to secrete Ig and rheumatoid factors (RF) upon polyclonal activation in vitro. B lymphocytes secreting IgM-RF and IgG-RF were enumerated using hemolytic plaque forming cell (PFC) assays. The sensitivity of RF-PFC formation was similar to that of a reverse PFC assay detecting all cells secreting Ig. Blood mononuclear cells from RA patients generated a median of 12 IgM-RF and 15 IgG-RF-secreting cells/10(6) after 6 days culture with pokeweed mitogen (PWM). Thus a median of approximately 0.5% of IgM- and IgG-secreting cells were identified as RF-PFC. Synovial fluid B lymphocytes co-cultured with autologous blood T lymphocytes in the presence of PWM generated higher numbers of RF-secreting cells; medians of 43 IgM-RF and 441 IgG-RF secreting cells/10(6) were found. Thus, 8-9% of IgM- and IgG-secreting cells from synovial fluid were identified as RF-PFC. In co-cultures containing synovial fluid T cells, PWM-induced RF secretion was low. The data indicate that both blood and SF B cells have the potential for RF secretion.
对类风湿性关节炎（RA）患者的淋巴细胞进行体外多克隆激活后分泌免疫球蛋白（Ig）和类风湿因子（RF）能力的检测。采用溶血空斑形成细胞（PFC）试验对分泌IgM-RF和IgG-RF的B淋巴细胞进行计数。RF-PFC形成的敏感性与检测所有分泌Ig细胞的反向PFC试验相似。类风湿性关节炎患者的血液单核细胞在与商陆有丝分裂原（PWM）培养6天后，产生的分泌IgM-RF细胞中位数为12个/10⁶，分泌IgG-RF细胞中位数为15个/10⁶。因此，约0.5%的分泌IgM和IgG的细胞被鉴定为RF-PFC。在PWM存在的情况下，与自体血液T淋巴细胞共培养的滑液B淋巴细胞产生了更多的分泌RF细胞；发现分泌IgM-RF细胞中位数为43个/10⁶，分泌IgG-RF细胞中位数为441个/10⁶。因此，滑液中8-9%的分泌IgM和IgG的细胞被鉴定为RF-PFC。在含有滑液T细胞的共培养物中，PWM诱导的RF分泌较低。数据表明，血液和滑液B细胞都有分泌RF的潜力。
