Bac Vo Hoai, Paulsen Berit Smestad, Truong Le Van, Koschella Andreas, Trinh Tat Cuong, Wold Christian Winther, Yogarajah Suthajini, Heinze Thomas
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam.
Department of Pharmacy, Section of Pharmaceutical Chemistry, University of Oslo, 0316 Oslo, Norway.
Polymers (Basel). 2019 Jul 22;11(7):1219. doi: 10.3390/polym11071219.
(Seem.) Guillaumin is a native tree in Vietnam. The water extract of the leaves from this tree gives a highly viscous product that has been used to heal wounds and treat inflammations. Our previous studies showed that the leaves of have a high content of polysaccharides. In this study, the structure and influence of the neutral polysaccharide from (PCA1) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells were investigated. The PCA1 isolated from is a galactan-type polysaccharide, containing galactose (77.0%), 3--methyl galactose (20.0%), and arabinose (3.0%). Linkage analysis of PCA1 showed that both the 3--methyl galactose and galactose were 1,4-linked. The presence of 3--methyl galactose units as part of the polysaccharide is important and can be used as a chemotaxonomic marker. The molecular weight of the PCA1 was 170 kDa. A PCA1 concentration of 30-40 μg/mL strongly inhibited TNFα, IL-1β, and IL-6 inflammatory cytokine production, and reactive oxygen species (ROS) release. PCA1 had inhibitory activities on pro-inflammatory cytokine and ROS release in LPS-stimulated mouse macrophages in vitro through MAPK signaling.
（似是。）吉约曼树是越南本土树木。该树树叶的水提取物能得到一种高粘性产物，已被用于伤口愈合和炎症治疗。我们之前的研究表明，（此处原文缺失树名）树叶含有高含量的多糖。在本研究中，对从（此处原文缺失树名）中提取的中性多糖（PCA1）对脂多糖（LPS）刺激的RAW264.7细胞的结构及影响进行了研究。从（此处原文缺失树名）中分离出的PCA1是一种半乳聚糖型多糖，含有半乳糖（77.0%）、3 - O - 甲基半乳糖（20.0%）和阿拉伯糖（3.0%）。PCA1的连接分析表明，3 - O - 甲基半乳糖和半乳糖均为1,4 - 连接。作为多糖一部分的3 - O - 甲基半乳糖单元的存在很重要，可作为化学分类学标记。PCA1的分子量为170 kDa。30 - 40μg/mL的PCA1浓度能强烈抑制TNFα、IL - 1β和IL - 6炎症细胞因子的产生以及活性氧（ROS）的释放。PCA1通过MAPK信号通路在体外对LPS刺激的小鼠巨噬细胞中的促炎细胞因子和ROS释放具有抑制活性。
