Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, P.O. Box. 45320, Islamabad, Pakistan.
Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, P.O. Box. 45320, Islamabad, Pakistan.
Phytomedicine. 2018 Jan 15;39:56-65. doi: 10.1016/j.phymed.2017.12.022. Epub 2017 Dec 20.
Nigella sativa, or commonly called black cumin is a small herb of family Ranunculaceae is a well-known medicinal plant but its effects on tissue mineral concentrations of animal bodies is unknown.
To study the effect of oral administration of fixed oil of black cumin seeds on tissues mineral content using laboratory rats as experimental model.
Experimental animals were exposed to two oral doses of seed oil (60 and 120 ml kg body weight). Short- and long term experiments lasted 24 h and 60 days respectively, with three replicates each.
Oil extracted from black cumin seeds was subjected to GC-MS to identify chemical components. Following the wet digestion in nitric acid, samples of whole blood and organs of rats were subjected to atomic absorption spectrophotometry for determination of elements concentrations. Data were compared statistically at p < .05.
Compared to control, Cr, Mn, Ni, Cu, Zn showed decrease, whereas Co, Na, Mg and K demonstrated increase, but Ca showed both increase and decrease in most of the tissues upon short term exposure to low and high doses of black cumin oil. During long term exposure, Cr, Fe, Mn, Cu exhibited decrease; Co, Na, Mg and Ca concentrations demonstrated an upregulation, whereas Ni and Zn showed increase and decrease in most of the tissues. Comparison of short term with long term experiments at low dose revealed increases in Fe, Zn, Cu, Mg, K and Ca, a decrease in Cr, Mn, Ni and Cu in most tissues, but both increase and decrease in Na. At high dose, an increase occurred in Fe, Ni, Zn, K, Ca, Mg, a decrease in Cr, while both increase and decrease in Cu, Co and Na concentrations.
Our study demonstrates that oral administration of black cumin seeds oil to laboratory rats significantly alters tissue trace elements and electrolytes concentrations. The study appears beneficial but indicates modulatory role of black cumin oil as regards mineral metabolism with far reaching implications in health and disease.
黑种草，又称黑孜然，是毛茛科的一种小型草本植物，是一种众所周知的药用植物，但它对动物体组织矿物质浓度的影响尚不清楚。
研究口服黑种草籽油对实验大鼠组织矿物质含量的影响。
实验动物暴露于两种口服剂量的种子油（60 和 120 毫升/公斤体重）。短期和长期实验分别持续 24 小时和 60 天，每个实验重复 3 次。
从黑种草种子中提取的油经过 GC-MS 分析以鉴定化学成分。在硝酸湿法消化后，对大鼠全血和器官样本进行原子吸收分光光度法测定元素浓度。在 p<0.05 时进行统计学比较。
与对照组相比，Cr、Mn、Ni、Cu、Zn 降低，而 Co、Na、Mg 和 K 增加，但在短期暴露于低剂量和高剂量黑种草油时，大多数组织中的 Ca 表现出增加和减少。在长期暴露期间，Cr、Fe、Mn、Cu 降低；Co、Na、Mg 和 Ca 浓度上调，而 Ni 和 Zn 则在大多数组织中增加和减少。与短期相比，在低剂量下的长期实验中，Fe、Zn、Cu、Mg、K 和 Ca 增加，Cr、Mn、Ni 和 Cu 减少，而 Na 增加和减少。在高剂量下，Fe、Ni、Zn、K、Ca、Mg 增加，Cr 减少，而 Cu、Co 和 Na 浓度增加和减少。
我们的研究表明，口服黑种草籽油对实验大鼠的组织微量元素和电解质浓度有显著影响。该研究显示了黑种草籽油的有益作用，但表明其对矿物质代谢具有调节作用，对健康和疾病具有深远的影响。
