Mukanova Zhansaya, Gudun Kristina, Elemessova Zarina, Khamkhash Laura, Ralchenko Ekaterina, Bukasov Rostislav
Chemistry Department, School of Science and Technology, Nazarbayev University.
Anal Sci. 2018;34(2):183-187. doi: 10.2116/analsci.34.183.
We demonstrated that a cost-efficient, easy to prepare, hybrid SERS substrate-gold nanoparticles (AuNPs) on untreated Al foil (AlF) can effectively detect pharmaceuticals, such as paracetamol and clinical biomarkers, like urea in artificial urine. The limit of detection (LOD) for paracetamol on AuNPs on AlF is superior (0.1 vs. 1 mM ) to the LOD reported for SERS detection of paracetamol in the literature. For SERS detection of urea in urine, AuNPs on both Al foil and Au film performed much better than AuNPs on glass, in terms of the concentration range, linearity and LOD. However, assay on AuNPs on AlF showed a better semi-logarithmic trendline with R = 0.98 than an assay on AuNPs on Au film with R = 0.94. They have comparable sensitivity with LOD 0.024 and 0.017 M, respectively. The limit of quantification (LOQ) of the former is 0.026 M, which makes it sufficient for the quantification of urea in urine at both normal and pathophysiological (0.03 - 0.15 M) concentration.
我们证明，一种经济高效、易于制备的混合表面增强拉曼光谱（SERS）基底——未处理铝箔（AlF）上的金纳米颗粒（AuNPs），能够有效检测药物，如对乙酰氨基酚，以及临床生物标志物，如人工尿液中的尿素。AlF上AuNPs对对乙酰氨基酚的检测限（LOD）（0.1 mM对1 mM）优于文献中报道的SERS检测对乙酰氨基酚的LOD。对于尿液中尿素的SERS检测，就浓度范围、线性和LOD而言，Al箔和Au膜上的AuNPs均比玻璃上的AuNPs表现更好。然而，AlF上AuNPs的检测显示出更好的半对数趋势线，R = 0.98，优于Au膜上AuNPs的检测，后者R = 0.94。它们具有相当的灵敏度，LOD分别为0.024 M和0.017 M。前者的定量限（LOQ）为0.026 M，这使其足以对正常和病理生理浓度（0.03 - 0.15 M）的尿液中的尿素进行定量。
