Chemistry Department, College of Science, United Arab Emirates University, P.O. Box, 15551, Al-Ain, United Arab Emirates.
Biology Department, College of Science, United Arab Emirates University, P.O. Box, 15551, Al-Ain, United Arab Emirates.
Sci Rep. 2018 Feb 12;8(1):2839. doi: 10.1038/s41598-018-21156-9.
We report the control of imazalil (IMZ) antifungal activity utilizing its non-covalent assembly with β-cyclodextrins (β-CD) and cucurbit[8]uril (CB8) macrocycles, as well as its stimuli-responsive disassembly with cadaverine. The NMR results are consistent with inclusion of a single IMZ molecule inside the cavities of either CB8 from its aromatic site or β-CD from its aliphatic end. Efficient complex formation with both host molecules and controlled released upon the addition of cadaverine is supported by NMR measurements. The stimuli-responsiveness of the same host-guest assemblies with cadaverine was validated against seven economically important plant pathogenic fungi which cause agriculturally important plant diseases across the globe. While loading the drug into macrocycles cavities suppressed its activity, subsequent adding of cadaverine efficiently restored it up. The results in the present paper enable researchers working in the area of mycology and plant pathology to inhibit or reduce the fungal growth on demand in order to control these economically important plant pathogenic fungi.
我们报告了利用其与β-环糊精（β-CD）和瓜环（CB8）大环的非共价组装来控制咪唑菌（IMZ）抗真菌活性，以及利用腐胺进行刺激响应的解组装。NMR 结果与从芳构化位点的 CB8 或从脂肪端的β-CD 内部包含单个 IMZ 分子的包合一致。NMR 测量支持与两种主体分子的有效络合形成以及在加入腐胺时的受控释放。针对在全球范围内引起农业重要植物病害的七种经济重要植物病原真菌，对同一主体客体组装物与腐胺的刺激响应性进行了验证。虽然将药物装入大环空腔中会抑制其活性，但随后添加腐胺可有效地将其恢复。本文的结果使从事真菌学和植物病理学领域的研究人员能够按需抑制或减少真菌生长，以控制这些经济上重要的植物病原真菌。
