Kavitha S, Khambhaty Yasmin, Chandra Babu N K
Leather Process Technology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, 600 020 India.
2Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Leather Research Institute (CSIR-CLRI), Adyar, Chennai, 600 020 India.
Indian J Microbiol. 2018 Mar;58(1):51-59. doi: 10.1007/s12088-017-0696-7. Epub 2017 Dec 9.
In the present investigation, a novel fungus was isolated from leather watch strap and identified as TANCK-1 by 18 s rRNA sequencing. The isolated fungus was evaluated against three structurally different fungicides such as 2-(thiocyanomethylthio)benzothiazole (TCMTB), potassium dimethyldithiocarbamate (KDDC) and 2,2-dibromo-3-nitrilopropionamide (DBNP) to control the fungal growth. Among these, TCMTB was found to effectively inhibit the growth at a minimum concentration of 31.2 µg/mL as compared to 1250 and 625 µg/mL for KDDC and DBNP respectively. Increased membrane permeability in the fungicide treated samples was evident from cellular release and decrease in cellular ergosterol content. Nevertheless, SEM analysis revealed a considerable change in fungicide treated mycelium with the collapse of hyphae structure and shrunken spores, which was observed to be very pronounced in KDDC and DBNP. Results indicated that all three biocides bring about inhibition through membrane damage with almost negligible effect on the cell wall.
Schematic diagram explaining the fungicidal action on TANCK-1.
在本研究中，从皮革表带中分离出一种新型真菌，并通过18 s rRNA测序鉴定为TANCK-1。对分离出的真菌进行了三种结构不同的杀菌剂的评估，即2-(硫氰基甲基硫代)苯并噻唑(TCMTB)、二甲基二硫代氨基甲酸钾(KDDC)和2,2-二溴-3-腈基丙酰胺(DBNP)，以控制真菌生长。其中，发现TCMTB在最低浓度31.2 μg/mL时能有效抑制生长，而KDDC和DBNP分别需要1250 μg/mL和625 μg/mL。杀菌剂处理样品中膜通透性增加，这从细胞释放和细胞麦角固醇含量降低中明显可见。然而，扫描电子显微镜分析显示，杀菌剂处理后的菌丝体有相当大的变化，菌丝结构塌陷，孢子萎缩，在KDDC和DBNP中这种现象非常明显。结果表明，所有这三种杀菌剂都是通过膜损伤来实现抑制作用的，对细胞壁的影响几乎可以忽略不计。
解释对TANCK-1的杀菌作用的示意图。
