MicroRNA-127 是否为急性胰腺炎肺损伤的新型生物标志物？

Is MicroRNA-127 a Novel Biomarker for Acute Pancreatitis with Lung Injury?

机构信息

Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, Chengdu, China.

Department of Gastroenterology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu Province, China.

出版信息

Dis Markers. 2017;2017:1204295. doi: 10.1155/2017/1204295. Epub 2017 Dec 24.

BACKGROUND AND AIMS

The aim of this study was to determine the expression of microRNA-127 (miR-127) in both rat models and patients of acute pancreatitis (AP) with lung injury (LI).

METHODS

Rats were administrated with retrograde cholangiopancreatography injection of 0.5% or 3.5% sodium taurocholate to induce AP with mild or severe LI and were sacrificed at 6, 12, and 24 h. Rats from the control group received a laparotomy only. Plasma from a prospective cohort of AP patients was collected. The levels of miR-127 in the tissues and plasma were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

RESULTS

The upregulation of miR-127 in the lungs of rats was detected in the groups of AP with severe LI at 6 h and 24 h, whereas it was scarcely detectable in plasma. In the pilot study that included 18 AP patients and 5 healthy volunteers, the plasma miR-127 level was significantly downregulated in AP patients with respiratory failure compared with the healthy volunteers ( = 0.014) and those without respiratory failure ( = 0.043).

CONCLUSION

miR-127 might serve as a potential marker for the identification of AP with LI.

背景与目的

本研究旨在检测 miR-127 在伴有肺损伤的急性胰腺炎（AP）大鼠模型和患者中的表达情况。

方法

采用逆行胰胆管注射 0.5%或 3.5%牛磺胆酸钠的方法建立伴有轻度或重度肺损伤的 AP 大鼠模型，并于造模后 6、12、24 h 处死大鼠。假手术组大鼠仅行开腹术。前瞻性收集 AP 患者的血浆标本。采用实时定量逆转录聚合酶链反应（qRT-PCR）检测组织和血浆中 miR-127 的水平。

结果

重度肺损伤 AP 大鼠模型在 6 h 和 24 h 时肺组织中 miR-127 的表达上调，而血浆中 miR-127 则几乎检测不到。在包括 18 例 AP 患者和 5 例健康志愿者的初步研究中，与健康志愿者（ = 0.014）和无呼吸衰竭的患者（ = 0.043）相比，伴有呼吸衰竭的 AP 患者的血浆 miR-127 水平显著降低（ = 0.014）。