Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, Chengdu, China.
Department of Gastroenterology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu Province, China.
Dis Markers. 2017;2017:1204295. doi: 10.1155/2017/1204295. Epub 2017 Dec 24.
The aim of this study was to determine the expression of microRNA-127 (miR-127) in both rat models and patients of acute pancreatitis (AP) with lung injury (LI).
Rats were administrated with retrograde cholangiopancreatography injection of 0.5% or 3.5% sodium taurocholate to induce AP with mild or severe LI and were sacrificed at 6, 12, and 24 h. Rats from the control group received a laparotomy only. Plasma from a prospective cohort of AP patients was collected. The levels of miR-127 in the tissues and plasma were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
The upregulation of miR-127 in the lungs of rats was detected in the groups of AP with severe LI at 6 h and 24 h, whereas it was scarcely detectable in plasma. In the pilot study that included 18 AP patients and 5 healthy volunteers, the plasma miR-127 level was significantly downregulated in AP patients with respiratory failure compared with the healthy volunteers ( = 0.014) and those without respiratory failure ( = 0.043).
miR-127 might serve as a potential marker for the identification of AP with LI.
本研究旨在检测 miR-127 在伴有肺损伤的急性胰腺炎（AP）大鼠模型和患者中的表达情况。
采用逆行胰胆管注射 0.5%或 3.5%牛磺胆酸钠的方法建立伴有轻度或重度肺损伤的 AP 大鼠模型，并于造模后 6、12、24 h 处死大鼠。假手术组大鼠仅行开腹术。前瞻性收集 AP 患者的血浆标本。采用实时定量逆转录聚合酶链反应（qRT-PCR）检测组织和血浆中 miR-127 的水平。
重度肺损伤 AP 大鼠模型在 6 h 和 24 h 时肺组织中 miR-127 的表达上调，而血浆中 miR-127 则几乎检测不到。在包括 18 例 AP 患者和 5 例健康志愿者的初步研究中，与健康志愿者（ = 0.014）和无呼吸衰竭的患者（ = 0.043）相比，伴有呼吸衰竭的 AP 患者的血浆 miR-127 水平显著降低（ = 0.014）。
miR-127 可能作为伴有肺损伤的 AP 的潜在标志物。
