Ren Hao, Yang Xiaomin, Yang Yongmei, Zhang Xiaoyu, Zhao Rui, Wei Ran, Zhang Xin, Zhang Yi
Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China.
Department of Laboratory, Yuhuangding Hospital, Qingdao University Medical College, Yantai, Shandong Province, China.
Oncotarget. 2017 Dec 21;9(4):4851-4861. doi: 10.18632/oncotarget.23585. eCollection 2018 Jan 12.
Brain cytoplasmic RNA 1 (BCYRN1), along non-coding RNA, plays a critical role in various diseases, including some cancers. However, the expression of BCYRN1 and its roles in gastric carcinoma (GC) still remain unidentified. Thus, this study employed RT-qPCR to detect expression of BCYRN1 in 85 paired GC samples and adjacent normal tissues, and performed studies to explore effects of BCYRN1 in GC cells on cell proliferation, apoptosis and migration. We found BCYRN1 was significantly upregulated in GC samples, and its expression was positively correlated with advanced TNM stage ( = 0.0012) and tumor size ( = 0.027). Functionally, BCYRN1 knockdown by siRNA could inhibit cell proliferation, induce G1/G0 cell cycle arrest, increase apoptosis and impair migratory ability of AGS cells. Moreover, the results of RT-qPCR and western blotting indicated that knockdown of BCYRN1 notably decreased the expression of epithelial cell adhesion molecules (EpCAM). Otherwise, overexpression of BCYRN1 in GC cells (BGC-823 and SGC-7901) could reverse the effects of BCYRN1 knockdown. Taken together, our data indicate for the first time that BCYRN1 acts as an oncogenic lncRNA in GC progression and may be a potential therapeutic target in GC.
脑细胞质RNA 1（BCYRN1）作为一种长链非编码RNA，在包括某些癌症在内的多种疾病中发挥着关键作用。然而，BCYRN1在胃癌（GC）中的表达及其作用仍不清楚。因此，本研究采用RT-qPCR检测85对GC样本及其癌旁正常组织中BCYRN1的表达，并进行相关研究以探讨BCYRN1对GC细胞增殖、凋亡和迁移的影响。我们发现BCYRN1在GC样本中显著上调，其表达与TNM分期晚期（P = 0.0012）和肿瘤大小（P = 0.027）呈正相关。在功能上，通过小干扰RNA（siRNA）敲低BCYRN1可抑制细胞增殖，诱导G1/G0期细胞周期阻滞，增加细胞凋亡并削弱AGS细胞的迁移能力。此外，RT-qPCR和蛋白质印迹结果表明，敲低BCYRN1可显著降低上皮细胞粘附分子（EpCAM）的表达。否则，在GC细胞（BGC-823和SGC-7901）中过表达BCYRN1可逆转敲低BCYRN1的作用。综上所述，我们的数据首次表明BCYRN1在GC进展中作为一种致癌长链非编码RNA发挥作用，可能是GC潜在的治疗靶点。
