Song Ji Eun, Su Jing, Noro Jennifer, Cavaco-Paulo Artur, Silva Carla, Kim Hye Rim
Department of Clothing and Textiles, Sookmyung Women's University, Cheongpa-ro-47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, South Korea.
Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
AMB Express. 2018 Feb 13;8(1):19. doi: 10.1186/s13568-018-0552-0.
In this work a process for the bio-coloration of bacterial cellulose (BC) membranes was developed. Laccase from Myceliophthora thermophila was immobilized onto BC membranes and retained up to 88% of residual activity after immobilization. Four compounds belonging to the flavonoids family were chosen to test the in situ polymerase activity of immobilized laccase. All the flavonoids were successfully polymerized by laccase giving rise to yellow, orange and dark brown oligomers which conferred color to the BC support. The optimal bio-coloration conditions were studied for two of the tested flavonoids, catechol and catechin, by varying the concentration and time of incubation. High color depth and resistance to washing were obtained for both compounds. The highly porous bacterial cellulose material demonstrated great performance as a bio-coloration support, in contrast to other materials cited in literature, like cotton or wool. The process developed is presented as an environmentally friendly alternative for bacterial cellulose bio-coloration and will contribute deeply for the development of new fashionable products within this material.
在这项工作中，开发了一种用于细菌纤维素（BC）膜生物染色的方法。嗜热毁丝霉的漆酶被固定在BC膜上，固定后保留了高达88%的残余活性。选择了四种属于黄酮类家族的化合物来测试固定化漆酶的原位聚合活性。所有黄酮类化合物都被漆酶成功聚合，产生了黄色、橙色和深棕色的低聚物，这些低聚物赋予了BC载体颜色。通过改变孵育浓度和时间，研究了两种测试黄酮类化合物（儿茶酚和儿茶素）的最佳生物染色条件。两种化合物都获得了高色深和耐洗性。与文献中提到的其他材料（如棉花或羊毛）相比，高度多孔的细菌纤维素材料作为生物染色载体表现出优异的性能。所开发的工艺被认为是一种用于细菌纤维素生物染色的环保替代方法，并将为这种材料内新型时尚产品的开发做出重要贡献。
