Takano Fumihide, Ihara Satomi, Kobayashi Kyoko, Ogiwara Tomoko, Yahagi Nobuo, Yahagi Remiko, Fushiya Shinji, Ohta Tomihisa, Yoshizaki Fumihiko
Kanazawa University, Kakuma-machi, Kanazawa , 920-1192, Japan.
Tohoku Pharmaceutical University, 4-1 Komatsushima 4-chome, Aoba-ku, Sendai 981-8558, Japan.
J Nat Med. 2006 Jul;60(3):243-247. doi: 10.1007/s11418-006-0034-6. Epub 2006 May 3.
In order to reveal the differences between Cordyceps, Paecilomyces (= Isaria) and Nomuraea, we collected seven entomogenous fungi grown in natural field and analyzed the profiles of water-soluble constituents derived from some different sources of Cordyceps, Paecilomyces and Nomuraea by determination using capillary electrophoresis. C. sinensis and C. kyushuensis showed similar peak clusters of protein migrated at 5-7, 8-9, and 12-20 min. The peak clusters obtained from N. atypicola was similar to those of C. sinensis and C. kyushuensis. The water-soluble constituent clusters of C. militaris migrated at 5-9 and 10-15 min were partly different from those of other Cordyceps. It was also revealed that the P. tenuipes and P. cicadae showed lesser peak clusters rather than Cordyceps. These results indicated that the profiles of protein of these entomogenous fungi by capillary electrophoretic analysis could serve as fingerprints for classification and medicinal quality control of Cordyceps.
为揭示冬虫夏草、拟青霉（= 棒束孢）和野村菌之间的差异，我们收集了7种在自然环境中生长的虫生真菌，并通过毛细管电泳测定分析了来自冬虫夏草、拟青霉和野村菌不同来源的水溶性成分谱。中华虫草菌和九州虫草菌在5 - 7分钟、8 - 9分钟和12 - 20分钟迁移的蛋白质显示出相似的峰簇。从异形野村菌获得的峰簇与中华虫草菌和九州虫草菌的相似。蛹虫草在5 - 9分钟和10 - 15分钟迁移的水溶性成分簇与其他冬虫夏草的部分不同。还发现细脚拟青霉和蝉拟青霉的峰簇比冬虫夏草少。这些结果表明，通过毛细管电泳分析这些虫生真菌的蛋白质谱可作为冬虫夏草分类和药用质量控制的指纹图谱。
