International Center for Diarrheal Diseases Research, Bangladesh, Dhaka, Bangladesh.
Stanford University, Stanford, California.
Am J Trop Med Hyg. 2018 Apr;98(4):1031-1038. doi: 10.4269/ajtmh.17-0408. Epub 2018 Feb 8.
Consumption of contaminated stored food can cause childhood diarrhea. Flies carry enteropathogens, although their contribution to food contamination remains unclear. We investigated the role of flies in contaminating stored food by collecting food and flies from the same households in rural Bangladesh. We selected 182 households with children ≤ 24 months old that had stored foods for later feeding at room temperature for ≥ 3 hours. We collected food samples and captured flies with fly tapes hung by the kitchen. We used the IDEXX Quanti-Tray System (Colilert-18 media; IDEXX Laboratories, Inc., Westbrook, ME) to enumerate with the most probable number (MPN) method. -positive IDEXX wells were analyzed by polymerase chain reaction for pathogenic genes (, , , , , , , , , and ). was detected in 61% (111/182) of food samples, with a mean of 1.1 log MPN/dry g. Fifteen samples (8%) contained pathogenic ; seven (4%) had enteropathogenic (EPEC) genes ( and/or ); and 10 (5%) had enteroaggregative genes ( and/or ). Of flies captured in 68 (37%) households, was detected in 41 (60%, mean 2.9 log MPN/fly), and one fly (1%) had an EPEC gene (). For paired fly-food samples, each log MPN increase in flies was associated with a 0.31 log MPN increase in stored food (95% confidence interval: 0.07, 0.55). In rural Bangladesh, flies possibly a likely route for fecal contamination of stored food. Controlling fly populations may reduce contamination of food stored for young children.
食用受污染的储存食物可能导致儿童腹泻。苍蝇携带肠道病原体，但它们对食物污染的贡献尚不清楚。我们通过从孟加拉国农村的同一家庭收集食物和苍蝇来研究苍蝇在污染储存食物方面的作用。我们选择了 182 户有≤24 个月大的儿童的家庭，这些家庭将食物存放在室温下≥3 小时后再喂食。我们收集了食物样本，并使用苍蝇胶带在厨房悬挂捕获苍蝇。我们使用 IDEXX Quanti-Tray 系统（Colilert-18 培养基； IDEXX 实验室公司，缅因州韦斯特布鲁克）用最可能数（MPN）法对进行计数。用 IDEXX 检测到阳性的 Wells 用聚合酶链反应分析致病性基因（、、、、、、、和）。在 111/182（61%）的食物样本中检测到，平均每克干重有 1.1 log MPN。15 个样本（8%）含有致病性；7 个（4%）含有肠致病性（EPEC）基因（和/或）；10 个（5%）含有肠聚集性（EAEC）基因（和/或）。在 68 个（37%）家庭中捕获的苍蝇中，有 41 个（60%，平均每只苍蝇 2.9 log MPN）检测到，其中 1 只苍蝇（1%）携带 EPEC 基因（）。对于配对的苍蝇-食物样本，苍蝇每增加 1 log MPN，储存食物中就会增加 0.31 log MPN（95%置信区间：0.07，0.55）。在孟加拉国农村，苍蝇可能是储存食物粪便污染的一个重要途径。控制苍蝇数量可能会减少对儿童储存食物的污染。
