通过非放射性原位杂交对与亨廷顿病相关的D4S10基因座进行精细定位。
Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization.
作者信息
Landegent J E, Jansen in de Wal N, Fisser-Groen Y M, Bakker E, van der Ploeg M, Pearson P L
出版信息
Hum Genet. 1986 Aug;73(4):354-7. doi: 10.1007/BF00279100.
PMID:2943662
Abstract
The chromosomal localization of a unique DNA fragment, closely linked to Huntington disease (HD), was assessed in situ by hybridization with 2-acetylaminofluorene (AAF) modified probes. In these experiments, a cosmid cloned genomic fragment (c5.5) was used for hybridization. Here we present evidence that confirms the mapping of the D4S10 locus to the p16 region of chromosome 4 and assigns it to the telomere of the short arm.
摘要
通过与2-乙酰氨基芴（AAF）修饰的探针进行原位杂交，评估了与亨廷顿病（HD）紧密连锁的一个独特DNA片段的染色体定位。在这些实验中，使用了一个黏粒克隆的基因组片段（c5.5）进行杂交。在此，我们提供的证据证实了D4S10基因座定位于4号染色体的p16区域，并将其定位在短臂的端粒处。
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