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化学修饰的核酸作为杂交实验中可免疫检测的探针。

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.

作者信息

Tchen P, Fuchs R P, Sage E, Leng M

出版信息

Proc Natl Acad Sci U S A. 1984 Jun;81(11):3466-70. doi: 10.1073/pnas.81.11.3466.

DOI:10.1073/pnas.81.11.3466
PMID:6374657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345529/
Abstract

Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative in an in vitro nonenzymatic reaction. The modified nucleic acids (ribo or deoxyribo, single or double stranded) are recognized by specific antibodies. They can be immunoprecipitated or used as probes in hybridization experiments and detected by immunochemical techniques.

摘要

核酸中的鸟嘌呤残基可在体外非酶促反应中通过用N-乙酰氧基-N-2-乙酰氨基芴及其7-碘衍生物处理来进行修饰。修饰后的核酸（核糖核酸或脱氧核糖核酸，单链或双链）可被特异性抗体识别。它们可被免疫沉淀，或用作杂交实验中的探针，并通过免疫化学技术进行检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88d0/345529/10195cee24dc/pnas00612-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88d0/345529/10195cee24dc/pnas00612-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88d0/345529/10195cee24dc/pnas00612-0214-a.jpg

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Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.化学修饰的核酸作为杂交实验中可免疫检测的探针。
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本文引用的文献

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Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate.二甲基亚砜溶液中DNA的热稳定性和复性：复性速率的加速
Biopolymers. 1980 Jul;19(7):1315-27. doi: 10.1002/bip.1980.360190708.
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Cloning and nucleotide sequence analysis of human embryonic zeta-globin cDNA.人胚胎ζ-珠蛋白cDNA的克隆与核苷酸序列分析
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Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
一种通过荧光DNA原位杂交鉴定阴道毛滴虫的新方法。
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1,N6-etheno deoxy and ribo adenosine and 3,N4-etheno deoxy and ribo cytidine phosphoramidites. Strongly fluorescent structures for selective introduction in defined sequence DNA and RNA molecules.1,N6-乙撑脱氧和核糖腺苷以及3,N4-乙撑脱氧和核糖胞苷亚磷酰胺。用于以特定序列选择性引入DNA和RNA分子的强荧光结构。
Nucleic Acids Res. 1994 Apr 11;22(7):1296-304. doi: 10.1093/nar/22.7.1296.
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Anti-DNA.RNA sera. Specificity tests and application in quantitative in situ hybridization.抗DNA.RNA血清。特异性测试及其在定量原位杂交中的应用。
Histochemistry. 1984;81(6):517-20. doi: 10.1007/BF00489529.
6
DNA probe specific for Legionella pneumophila.嗜肺军团菌特异性DNA探针。
J Clin Microbiol. 1985 Mar;21(3):431-7. doi: 10.1128/jcm.21.3.431-437.1985.
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Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.时间分辨荧光分析法：一种定量检测DNA杂交体的灵敏方法。
Nucleic Acids Res. 1986 Jan 24;14(2):1017-28. doi: 10.1093/nar/14.2.1017.
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Appl Environ Microbiol. 1985 Jun;49(6):1374-8. doi: 10.1128/aem.49.6.1374-1378.1985.
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Nucleic Acids Res. 1985 Apr 25;13(8):2789-802. doi: 10.1093/nar/13.8.2789.
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EMBO J. 1983;2(6):817-22. doi: 10.1002/j.1460-2075.1983.tb01508.x.
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