Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, UrbanaIL 61801, United States.
Biotechnol J. 2018 Sep;13(9):e1700601. doi: 10.1002/biot.201700601. Epub 2018 Apr 18.
Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
得益于其易用性、模块化和可扩展性，簇状规律间隔短回文重复序列 (CRISPR) 系统已越来越多地用于酿酒酵母的设计和工程改造，酿酒酵母是工业生物技术中最受欢迎的宿主之一。本综述总结了该颠覆性技术在代谢工程应用中的最新发展，包括 CRISPR 介导的基因敲除和敲入以及转录激活和干扰。更重要的是，强调了多功能 CRISPR 系统，该系统结合了组合代谢工程的增益和失活调节。
