Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Metab Eng. 2018 Nov;50:85-108. doi: 10.1016/j.ymben.2018.04.011. Epub 2018 Apr 25.
Metabolic engineering aims to develop efficient cell factories by rewiring cellular metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae has been extensively engineered to produce a wide variety of products at high levels from various feedstocks. In this review, we summarize the recent development of metabolic engineering approaches to modulate yeast metabolism with representative examples. Particularly, we highlight new tools for biosynthetic pathway optimization (i.e. combinatorial transcriptional engineering and dynamic metabolic flux control) and genome engineering (i.e. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system based genome engineering and RNA interference assisted genome evolution) to advance metabolic engineering in yeast. We also discuss the challenges and perspectives for high throughput metabolic engineering.
代谢工程旨在通过重新布线细胞代谢来开发高效的细胞工厂。作为最常用的细胞工厂之一，酿酒酵母已被广泛工程化，以从各种原料中高水平生产各种产品。在这篇综述中，我们总结了代谢工程方法的最新进展，用代表性实例来调节酵母代谢。特别是，我们强调了生物合成途径优化的新工具（即组合转录工程和动态代谢通量控制）和基因组工程（即基于成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关（Cas）系统的基因组工程和 RNA 干扰辅助基因组进化），以推进酵母中的代谢工程。我们还讨论了高通量代谢工程的挑战和展望。
