Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China.
Vet Res. 2018 Feb 13;49(1):16. doi: 10.1186/s13567-018-0513-2.
Cell-penetrating peptide (CPP) is a promising cargo for delivering bioactive molecules. In this study, the N terminus of VP1 from chicken anemia virus, designated as CVP1, was found to carry enriched arginine residues with α-helix. By confocal imaging, flow cytometry and MTT assay, we identified CVP1 as a novel, safe and efficient CPP. CVP1-FITC peptide could entry different types of cells tested with dose dependence, but without cytotoxic effects. Compared with TAT-FITC peptide, the CVP1-FITC peptide showed much higher cell-penetrating activity. Moreover, CVP1 could successfully deliver β-glycosidase, poly (I:C) and plasmid into HCT116 cells. Inhibitors and temperature sensitivity analysis further indicated that the cell-penetrating activity of CVP1 was based on ATP-dependent and caveolae-mediated endocytosis. All these data demonstrate that CVP1 has efficient cell-penetrating activity and great potential for developing a novel delivery vector.
细胞穿透肽（CPP）是一种很有前途的运载生物活性分子的载体。在这项研究中，我们发现来自鸡贫血病毒的 VP1 的 N 端，被命名为 CVP1，携带富含精氨酸残基的α-螺旋。通过共聚焦成像、流式细胞术和 MTT 检测，我们鉴定出 CVP1 是一种新型、安全、高效的 CPP。CVP1-FITC 肽可以剂量依赖地进入不同类型的细胞，但没有细胞毒性作用。与 TAT-FITC 肽相比，CVP1-FITC 肽具有更高的细胞穿透活性。此外，CVP1 能够成功地将β-葡糖苷酶、聚（I:C）和质粒递送至 HCT116 细胞。抑制剂和温度敏感性分析进一步表明，CVP1 的细胞穿透活性是基于 ATP 依赖性和小窝蛋白介导的内吞作用。所有这些数据表明 CVP1 具有有效的细胞穿透活性，为开发新型递药载体提供了巨大的潜力。
