Subhan Fazli, Shahzad Raheem, Tauseef Isfahan, Haleem Kashif Syed, Rehman Atta-Ur, Mahmood Sajid, Lee In-Jung
Department of Microbiology, Hazara University, Mansehra, Pakistan.
School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
PeerJ. 2018 Jan 12;6:e4245. doi: 10.7717/peerj.4245. eCollection 2018.
Beaches are recreational spots for people. However, beach sand contains harmful microbes that affect human health, and there are no established methods for either sampling and identifying beach-borne pathogens or managing the quality of beach sand.
This study was conducted with the aim of improving human safety at beaches and augmenting the quality of the beach experience. Beach sand was used as a resource to isolate bacteria due to its distinctive features and the biodiversity of the beach sand biota. A selected bacterial isolate termed FSRS was identified as using 16S rRNA sequencing and phylogenetic analysis, and the sequence was deposited in the NCBI GenBank database under the accession number MF599548. The isolated bacterium was cultured in Luria-Bertani growth medium, and a crude extract was prepared using ethyl acetate to examine the potential pathogenic effect of on human skin. A human skin keratinocyte cell line (HaCaT) was used to assess cell adhesion, cell viability, and cell proliferation using a morphological analysis and a WST-1 assay.
The crude extract inhibited cell adhesion and decreased cell viability in HaCaT cells. We concluded that the crude extract of FSRS had a strong pathological effect on human skin cells.
Beach visitors frequently get skin infections, but the exact cause of the infections is yet to be determined. The beach sand bacterium may, therefore, be responsible for some of the dermatological problems experienced by people visiting the beach.
海滩是人们的休闲场所。然而，海滩沙子中含有影响人类健康的有害微生物，目前尚无既定的方法来采样和鉴定源自海滩的病原体或管理海滩沙子的质量。
本研究旨在提高海滩上的人类安全性并提升海滩体验的质量。由于海滩沙子的独特特征和海滩沙子生物群的生物多样性，海滩沙子被用作分离细菌的资源。使用16S rRNA测序和系统发育分析将一种选定的细菌分离株命名为FSRS进行鉴定，该序列已保存在NCBI GenBank数据库中，登录号为MF599548。将分离出的细菌在Luria-Bertani生长培养基中培养，并用乙酸乙酯制备粗提物，以检查其对人类皮肤的潜在致病作用。使用人皮肤角质形成细胞系（HaCaT），通过形态学分析和WST-1测定来评估细胞粘附、细胞活力和细胞增殖。
粗提物抑制了HaCaT细胞的细胞粘附并降低了细胞活力。我们得出结论，FSRS的粗提物对人类皮肤细胞具有强烈的病理作用。
海滩游客经常会发生皮肤感染，但感染的确切原因尚未确定。因此，海滩沙子细菌可能是导致前往海滩的人出现一些皮肤问题的原因。
