Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Agriculture of Yangtze University, Jingzhou, Hubei Province, China.
Insect Sci. 2019 Aug;26(4):620-634. doi: 10.1111/1744-7917.12576. Epub 2018 Mar 26.
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice, copulation, reproduction and other behaviors. Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information. In this study, we present a genome-wide and transcriptome-wide investigation of the OBPs and CSPs in B. tabaci MEAM1 (Middle East-Asia Minor1 species). Eight OBP and 19 CSP genes were identified that covered all previous sequences. Phylogenetic analyses showed that the CSP genes had a lineage-specific expansion (BtabBCSP1, BtabBCSP3, BtabBCSP13, BtabBCSP17, BtabBCSP18 and BtabBCSP19). Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real-time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages of B. tabaci MEAM1. Five OBP genes and 11 CSP genes significantly differed between males and females; four of the 19 CSP genes were highly expressed in adults, while two were highly expressed in nymphs. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MEAM1 adults were analyzed by qPCR. Four OBP genes found in B. tabaci MEAM1 were highly expressed in the head. Conversely, only two CSPs were enriched in the head, while the other six CSPs were specifically expressed in other tissues. Our results provide a foundation for future research on OBPs and CSPs in B. tabaci.
昆虫的气味结合蛋白（OBPs）和化学感受蛋白（CSPs）被认为在嗅觉识别中发挥作用，影响宿主选择、交配、繁殖和其他行为。先前对白粉虱 Bemisia tabaci 的 OBPs 和 CSPs 的描述往往没有或提供不完整的遗传信息。在这项研究中，我们对 B. tabaci MEAM1（中东-亚洲小种群 1 物种）进行了全基因组和转录组范围内的 OBPs 和 CSPs 研究。鉴定了 8 个 OBP 和 19 个 CSP 基因，涵盖了所有以前的序列。系统发育分析表明，CSP 基因具有谱系特异性扩张（BtabBCSP1、BtabBCSP3、BtabBCSP13、BtabBCSP17、BtabBCSP18 和 BtabBCSP19）。通过转录组测序和定量实时聚合酶链反应（qPCR）进行的 OBPs 和 CSPs 表达谱分析表明，B. tabaci MEAM1 的发育阶段之间的表达模式不同。5 个 OBP 基因和 11 个 CSP 基因在雄性和雌性之间存在显著差异；19 个 CSP 基因中有 4 个在成虫中高度表达，而有 2 个在若虫中高度表达。通过 qPCR 分析了 B. tabaci MEAM1 成虫不同组织中 OBPs 和 CSPs 的表达谱。在 B. tabaci MEAM1 中发现的 4 个 OBP 基因在头部高度表达。相反，只有 2 个 CSPs 在头部富集，而其他 6 个 CSPs 则特异性表达在其他组织中。我们的研究结果为未来研究 B. tabaci 的 OBPs 和 CSPs 提供了基础。
