Forestry College, Henan University of Science and Technology, Luoyang, 471000, China.
Institute of Plant Nutrition and Resource Environment, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Sci Rep. 2018 May 17;8(1):7803. doi: 10.1038/s41598-018-26137-6.
Tropidothorax elegans Distant (Hemiptera: Lygaeidae) is an insect pest that inflicts damage to vegetables and flowering plants across China. The olfactory system regulates insect behavior, such as feeding, mating, oviposition and predator avoidance. Odorant-binding proteins (OBPs) and the chemosensory proteins (CSPs) are two groups of small soluble proteins that initiate olfactory signal transduction in insects. In this study, we generated antennal transcriptomes of male and female T. elegans, and identified 19 putative OBP (14 classic OBPs and five plus-C OBPs) and seven CSP genes. Through real-time quantitative PCR analysis, we found that 14 of the 19 OBP genes were highly expressed in the antennae of both adult females and males, and 3 OBP genes were expressed higher in the antennae of males than females. Some OBP genes were also highly expressed in the legs or wings. Three CSP genes were highly expressed in the antennae of both sexes, and TeleCSP7 showed higher expression in male antennae compare to females. Interestingly, one CSP gene, TeleCSP2, was expressed in all olfactory tissues. Our results provide molecular insights into further investigating of the olfactory system of an important plant pest, T. elegans.
优雅盲蝽（Tropidothorax elegans Distant）（半翅目：盲蝽科）是一种在中国为害蔬菜和花卉的害虫。昆虫的嗅觉系统调节着其取食、交配、产卵和躲避捕食者等行为。气味结合蛋白（OBP）和化学感受蛋白（CSP）是两类能引发昆虫嗅觉信号转导的小的可溶性蛋白。本研究中，我们生成了雌雄优雅盲蝽的触角转录组，并鉴定了 19 个假定的 OBP（14 个经典 OBP 和 5 个加-C OBP）和 7 个 CSP 基因。通过实时定量 PCR 分析，我们发现 19 个 OBP 基因中的 14 个在雌雄成虫触角中高表达，其中 3 个在雄虫触角中的表达量高于雌虫。一些 OBP 基因在腿或翅膀中也有高表达。3 个 CSP 基因在雌雄触角中高表达，TeleCSP7 在雄虫触角中的表达量高于雌虫。有趣的是，一个 CSP 基因 TeleCSP2 在所有嗅觉组织中都有表达。本研究为进一步研究重要植物害虫优雅盲蝽的嗅觉系统提供了分子见解。
