Ohkura N, Ohnishi K, Taniguchi M, Nakayama A, Usuba Y, Fujita M, Fujii A, Ishibashi K, Baba K, Atsumi G
Pharmazie. 2016 Nov 2;71(11):651-654. doi: 10.1691/ph.2016.6678.
Angelica keiskei Koidzumi (Ashitaba) is a traditional folk medicine that is also regarded in Japan as a health food with potential antithrombotic properties. The ability of the major chalcones, xanthoangelol (XA) and 4-hydroxyderricin (4-HD) extracted from Ashitaba roots to inhibit platelet aggregation activity in vitro was recently determined. However, the anti-platelet activities of Ashitaba chalcones in vivo have remained unclear. The present study examines the anti-platelet effects of Ashitaba exudate and its constituent chalcones using mouse tail-bleeding models that reflect platelet aggregation in vivo. Ashitaba exudate and the major chalcone subtype XA, suppressed the lipopolysaccharide (LPS)-induced shortening of mouse tail bleeding. However, trace amounts of other Ashitaba chalcone subtypes including xanthoangelols B (XB), D (XD), E (XE) and F (XF) did not affect tail bleeding. These results suggest that the major chalcone subtype in Ashitaba, XA, has anti-platelet-activities in vivo.
明日叶是一种传统民间药物，在日本也被视为具有潜在抗血栓特性的健康食品。最近测定了从明日叶根中提取的主要查尔酮——黄当归醇（XA）和4-羟基德里辛（4-HD）在体外抑制血小板聚集活性的能力。然而，明日叶查尔酮在体内的抗血小板活性仍不清楚。本研究使用反映体内血小板聚集的小鼠尾部出血模型，研究了明日叶渗出液及其成分查尔酮的抗血小板作用。明日叶渗出液和主要查尔酮亚型XA可抑制脂多糖（LPS）诱导的小鼠尾部出血时间缩短。然而，痕量的其他明日叶查尔酮亚型，包括黄当归醇B（XB）、D（XD）、E（XE）和F（XF），对尾部出血没有影响。这些结果表明，明日叶中的主要查尔酮亚型XA在体内具有抗血小板活性。
