College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
Ningxia Zhihong Biotechnology Company, Kaiyuan east road 29, Yinchuan, Ningxia, 750000, China.
Cell Stress Chaperones. 2018 Jul;23(4):735-748. doi: 10.1007/s12192-018-0885-2. Epub 2018 Feb 13.
Heat stress is exacerbated by global warming and affects human and animal health, leading to heart damage caused by imbalances in reactive oxygen species (ROS) and the antioxidant system, acid-base chemistry, electrolytes and respiratory alkalosis. Vitamin C scavenges excess ROS, and sodium bicarbonate maintains acid-base and electrolyte balance, and alleviates respiratory alkalosis. Herein, we explored the ability of vitamin C alone and in combination with equimolar sodium bicarbonate (Vitamin C-Na) to stimulate endogenous antioxidants and heat shock proteins (HSPs) to relieve heat stress in H9C2 cells. Control, vitamin C (20 μg/ml vitamin C for 16 h) and vitamin C-Na (20 μg/ml vitamin C-Na for 16 h) groups were heat-stressed for 1, 3 or 5 h. Granular and vacuolar degeneration, karyopyknosis and damage to nuclei and mitochondria were clearly reduced in treatment groups, as were apoptosis, lactate dehydrogenase activity and ROS and malondialdehyde levels, while superoxide dismutase activity was increased. Additionally, CRYAB, Hsp27, Hsp60 and Hsp70 mRNA levels were upregulated at 3 h (p < 0.01), and protein levels were increased for CRYAB at 0 h (p < 0.05) and 1 h (p < 0.01), and for Hsp70 at 3 and 5 h (p < 0.01). Thus, pre-treatment with vitamin C or vitamin C-Na might protect H9C2 cells against heat damage by enhancing the antioxidant ability and upregulating CRYAB and Hsp70.
热应激会因全球变暖而加剧，并影响人类和动物的健康，导致活性氧（ROS）和抗氧化系统、酸碱化学、电解质和呼吸性碱中毒失衡引起的心脏损伤。维生素 C 可以清除多余的 ROS，而碳酸氢钠则可以维持酸碱和电解质平衡，并缓解呼吸性碱中毒。在这里，我们探索了单独使用维生素 C 以及与等摩尔碳酸氢钠（维生素 C-Na）联合使用以刺激内源性抗氧化剂和热休克蛋白（HSPs）来缓解 H9C2 细胞热应激的能力。对照组、维生素 C（20μg/ml 维生素 C 处理 16h）和维生素 C-Na（20μg/ml 维生素 C-Na 处理 16h）组分别进行 1、3 或 5h 的热应激。在治疗组中，颗粒状和空泡状变性、核固缩和核损伤以及线粒体损伤明显减少，同时细胞凋亡、乳酸脱氢酶活性、ROS 和丙二醛水平降低，而超氧化物歧化酶活性增加。此外，CRYAB、Hsp27、Hsp60 和 Hsp70 的 mRNA 水平在 3h 时上调（p<0.01），CRYAB 的蛋白水平在 0h（p<0.05）和 1h（p<0.01）时上调，Hsp70 的蛋白水平在 3h 和 5h 时上调（p<0.01）。因此，维生素 C 或维生素 C-Na 的预处理可能通过增强抗氧化能力和上调 CRYAB 和 Hsp70 来保护 H9C2 细胞免受热损伤。
