Lin Xiao-Ning, Tian Xinli, Li Wang, Sun Jin, Wei Feng, Feng Wei, Huang Zhi-Chun, Tian Xin-Hua
Department of Neurosurgery, Zhongshan Hospital of Xiamen University, Xiamen 361004, China.
Department of Cardiovascular Disease, Chinese People's Liberation Army General Hospital of Beijing Military Region, Beijing 100000, China.
J Nanosci Nanotechnol. 2018 Apr 1;18(4):2325-2329. doi: 10.1166/jnn.2018.14379.
Gliomas are the most common type of intracranial malignant tumor; however, current treatment approaches are often ineffective due to limited penetration of genes or drugs through the blood-brain barrier (BBB). Here we describe the synthesis of gelatin-siloxane nanoparticles (GS NPs) as candidate gene carriers through a two-step sol-gel process. To increase the efficiency of glioma targeting, human immunodeficiency virus-derived Tat, tumor-targeting aptamer (TTA)1, and polyethylene glycol (PEG) were conjugated to the GS NPs to generate Tat-TTA1-PEG-GS NPs. In vivo imaging revealed that these modified NPs not only evaded capture by the reticulo-endothelial system, but were able to cross the BBB to reach gliomas. Our results suggest that Tat-TTA1-PEG-GS NPs are a new type of non-viral vector that can deliver therapeutic DNA or drugs for highly efficient glioma treatment.
神经胶质瘤是最常见的颅内恶性肿瘤类型；然而，由于基因或药物透过血脑屏障（BBB）的能力有限，目前的治疗方法往往无效。在此，我们描述了通过两步溶胶-凝胶法合成明胶-硅氧烷纳米颗粒（GS NPs）作为候选基因载体。为了提高神经胶质瘤靶向效率，将源自人类免疫缺陷病毒的Tat、肿瘤靶向适体（TTA）1和聚乙二醇（PEG）与GS NPs偶联，以生成Tat-TTA1-PEG-GS NPs。体内成像显示，这些修饰的纳米颗粒不仅能逃避网状内皮系统的捕获，还能够穿过血脑屏障到达神经胶质瘤。我们的结果表明，Tat-TTA1-PEG-GS NPs是一种新型非病毒载体，可递送治疗性DNA或药物用于高效的神经胶质瘤治疗。
