Chen Xiang-Dong, Li Shen, Du Bo, Dong Yang, Wang Ze-Hao, Guo Guang-Can, Sun Fang-Wen
Opt Lett. 2018 Feb 15;43(4):699-702. doi: 10.1364/OL.43.000699.
Multiphoton microscopy (MPM) has been widely used for three-dimensional biological imaging. Here, based on the photon-induced charge state conversion process, we demonstrated a low-power high-resolution MPM with a nitrogen vacancy (NV) center in diamond. Continuous wave green and orange lasers were used to pump and detect the two-photon charge state conversion, respectively. The power of the laser for multiphoton excitation was 40 μW. Both the axial and lateral resolutions were improved approximately 1.5 times compared with confocal microscopy. The results can be used to improve the resolution of the NV center-based quantum sensing and biological imaging.
多光子显微镜（MPM）已被广泛用于三维生物成像。在此，基于光子诱导的电荷态转换过程，我们展示了一种在金刚石中具有氮空位（NV）中心的低功率高分辨率MPM。连续波绿色和橙色激光器分别用于泵浦和检测双光子电荷态转换。用于多光子激发的激光功率为40 μW。与共聚焦显微镜相比，轴向和横向分辨率均提高了约1.5倍。这些结果可用于提高基于NV中心的量子传感和生物成像的分辨率。
