College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang 310018, China.
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang 310018, China.
Int J Biol Macromol. 2018 Jul 1;113:543-549. doi: 10.1016/j.ijbiomac.2018.02.059. Epub 2018 Feb 11.
An efficient acid-hydrolysis method was developed and optimized for the hydrolyses of polysaccharide from Auricularia auricula with the ABTS· scavenging ability as the detective marker. Based on the single factor experimental results, Box-Behnken design (BBD) were applied for the optimization of acid-hydrolysis conditions. The possible antioxidant mechanism of the hydrolyses (AAPs-F) in vivo was performed using the C. elegans model. The acid-hydrolysis conditions were found to be the optimal hydrolyzing time 2.78h (166.8min), hydrolyzing temperature 95.04°C and the acid concentration 14.03mol/L, respectively. Under the optimal acid-hydrolysis conditions, the ABTS· scavenging ability of AAPs-H was 97.94±0.87%, which was well matched with the predicted value (99.77%) of the BBD model. AAPs-F was the main fraction of AAPs-H separated through Sephadex G-10 as the stationary phase. AAPs-F was a kind of heteropolysaccharide and comprised of glucose, galactose and fucose with the molar ratio of 50:1:2. The molecular weight of AAPs-F was 143.15kDa. AAPs-F showed a remarkable protective effect to the injury induced by hydrogen peroxide or paraquat (p<0.01), and it could up-regulate stress-resistance related enzymes including superoxide dismutase (SOD) by 109.74% and CAT by 106.84% at concentration of 0.2mg/mL in C. elegans.
一种高效的酸水解方法被开发并优化，用于以 ABTS·清除能力为检测标记物的银耳多糖水解。基于单因素实验结果，采用 Box-Behnken 设计（BBD）对酸水解条件进行优化。利用秀丽隐杆线虫模型研究了水解物（AAPs-F）的体内可能的抗氧化机制。发现酸水解条件的最佳水解时间为 2.78h（166.8min），水解温度为 95.04°C，酸浓度为 14.03mol/L。在最佳酸水解条件下，AAPs-H 的 ABTS·清除能力为 97.94±0.87%，与 BBD 模型的预测值（99.77%）非常吻合。AAPs-F 是通过 Sephadex G-10 作为固定相分离的 AAPs-H 的主要部分。AAPs-F 是一种杂多糖，由葡萄糖、半乳糖和岩藻糖组成，摩尔比为 50:1:2。AAPs-F 的分子量为 143.15kDa。AAPs-F 对过氧化氢或百草枯引起的损伤表现出显著的保护作用（p<0.01），并且在浓度为 0.2mg/mL 时，它可以使秀丽隐杆线虫中的应激相关酶如超氧化物歧化酶（SOD）上调 109.74%，CAT 上调 106.84%。
