Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Curr Opin Biotechnol. 2018 Oct;53:158-163. doi: 10.1016/j.copbio.2017.12.020. Epub 2018 Feb 13.
Continuous directed evolution is the 'while loop' of synthetic biology, autonomous cycles of mutation, selection, and self-replication that can lead to the rapid development of industrially relevant organisms, pathways, or molecules. Although this engineering strategy requires particular mutagenesis methods and well-defined selections, recent advances have facilitated its implementation. Control over selection pressure has been augmented by novel cost-effective continuous culturing devices with open source designs. New in vivo targeted mutagenesis methods have enabled continuous directed protein evolution in various organisms. Furthermore, advances in automation have enabled rational, semi-continuous directed evolution strategies that may yield fewer artefacts or parasites. Overall, continuous directed evolution is persistently demonstrating its capacity to rapidly generate biotechnologically valuable strains and proteins.
连续定向进化是合成生物学的“while 循环”，是突变、选择和自我复制的自主循环，可导致具有工业相关性的生物体、途径或分子的快速发展。尽管这种工程策略需要特殊的诱变方法和明确的选择，但最近的进展已经促进了它的实施。通过具有开源设计的新型经济高效的连续培养装置，增强了对选择压力的控制。新的体内靶向诱变方法使各种生物体中的连续定向蛋白质进化成为可能。此外，自动化的进步使合理的、半连续的定向进化策略成为可能，这些策略可能产生更少的人工制品或寄生虫。总的来说，连续定向进化持续证明了其快速产生具有生物技术价值的菌株和蛋白质的能力。
