Baran D, Moreau J L, Thèze J
Ann Inst Pasteur Immunol. 1986 Jul-Aug;137D(1):23-31.
Using two purified monoclonal antibodies which recognize distinct epitopes of the murine IL2 receptor, we devised a new radioimmunoassay for the detection of free IL2 receptor in lymphocyte culture supernatants. Murine cell lines of both helper and cytotoxic phenotypes produce free IL2 receptor. There appears to be a quantitative relationship between membrane IL2 receptor expression and the presence of free IL2 receptor. The latter molecule may have a biological role, possibly that of a negative signal for lymphocyte growth and/or that of an IL2 transport protein.
利用两种可识别小鼠白细胞介素-2（IL2）受体不同表位的纯化单克隆抗体，我们设计了一种新的放射免疫分析法，用于检测淋巴细胞培养上清液中的游离IL2受体。具有辅助和细胞毒性表型的小鼠细胞系均可产生游离IL2受体。膜IL2受体表达与游离IL2受体的存在之间似乎存在定量关系。后一种分子可能具有生物学作用，可能是作为淋巴细胞生长的负信号和/或作为一种IL2转运蛋白。
