Pacheco-Soares Thaís, de Oliveira Carvalho André, da Silva Araújo Jucélia, de Souza Giliane da Silva, Machado Olga L T
Biosciences and Biotechnology, Northern State University of Rio de Janeiro - Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, 28013602, Brazil.
Biosciences and Biotechnology, Northern State University of Rio de Janeiro - Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, 28013602, Brazil
Biosci Rep. 2018 Feb 14;38(2). doi: 10.1042/BSR20171245.
Ric c1, an allergenic protein from , is an insect α-amylase inhibitor that has become an occupational allergen. Ric c1 can cross-react with allergens from wheat, soybean, peanut, shrimp, fish, gluten, house dust, tobacco, and air fungus, thereby amplifying the concern and risks caused by allergens. Two continuous IgE-binding epitopes were identified in Ric c1, both containing glutamic acid residues involved in IgE-binding and allergic challenges. We produced recombinant Ric c1 (rRic c1) in , using primers from foliar DNA, and a mutant (Glu-Leu) recombinant protein (mrRic c1) in the same system using synthetic genes. rRic c1 preserved both allergenic and α-amylase inhibitory properties, and mrRic c1 drastically reduced allergenic properties. These results can help to establish meaningful relationships between structure, defense and allergenicity, important steps for producing engineered plants and developing new approaches for immunotherapy.
Ric c1是来自[具体来源未明确]的一种变应原蛋白，是一种昆虫α-淀粉酶抑制剂，已成为一种职业性变应原。Ric c1可与小麦、大豆、花生、虾、鱼、麸质、屋尘、烟草和空气真菌中的变应原发生交叉反应，从而加剧了由这些变应原引起的担忧和风险。在Ric c1中鉴定出两个连续的IgE结合表位，两者均含有参与IgE结合和过敏激发的谷氨酸残基。我们使用来自叶片[具体来源未明确]DNA的引物在[具体系统未明确]中生产重组Ric c1（rRic c1），并在同一系统中使用合成基因生产突变体（Glu-Leu）重组蛋白（mrRic c1）。rRic c1保留了变应原性和α-淀粉酶抑制特性，而mrRic c1的变应原性大幅降低。这些结果有助于建立结构、防御和变应原性之间的有意义关系，这是生产工程植物和开发免疫治疗新方法的重要步骤。
