Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Chem Soc Rev. 2018 Mar 21;47(6):1996-2019. doi: 10.1039/c7cs00573c. Epub 2018 Feb 15.
Modern optical detection technology plays a critical role in current clinical detection due to its high sensitivity and accuracy. However, higher requirements such as extremely high detection sensitivity have been put forward due to the clinical needs for the early finding and diagnosing of malignant tumors which are significant for tumor therapy. The technology of isothermal amplification with nucleic acids opens up avenues for meeting this requirement. Recent reports have shown that a nucleic acid amplification-assisted modern optical sensing interface has achieved satisfactory sensitivity and accuracy, high speed and specificity. Compared with isothermal amplification technology designed to work completely in a solution system, solid biosensing interfaces demonstrated better performances in stability and sensitivity due to their ease of separation from the reaction mixture and the better signal transduction on these optical nano-biosensing interfaces. Also the flexibility and designability during the construction of these nano-biosensing interfaces provided a promising research topic for the ultrasensitive detection of cancer diseases. In this review, we describe the construction of the burgeoning number of optical nano-biosensing interfaces assisted by a nucleic acid amplification strategy, and provide insightful views on: (1) approaches to the smart fabrication of an optical nano-biosensing interface, (2) biosensing mechanisms via the nucleic acid amplification method, (3) the newest strategies and future perspectives.
现代光学检测技术由于其灵敏度和准确性高，在当前的临床检测中发挥着关键作用。然而，由于临床需要早期发现和诊断恶性肿瘤，因此对检测灵敏度提出了更高的要求，这对肿瘤治疗具有重要意义。核酸等温扩增技术为满足这一要求开辟了道路。最近的报道表明，核酸扩增辅助的现代光学传感界面已经实现了令人满意的灵敏度和准确性、高速和特异性。与旨在完全在溶液系统中工作的等温扩增技术相比，由于易于从反应混合物中分离以及在这些光学纳米生物传感界面上更好的信号转导，固体生物传感界面在稳定性和灵敏度方面表现出更好的性能。此外，在构建这些纳米生物传感界面时的灵活性和可设计性为癌症等疾病的超灵敏检测提供了一个有前途的研究课题。在本文中，我们描述了由核酸扩增策略辅助的新兴数量的光学纳米生物传感界面的构建，并提供了以下方面的深刻见解：（1）智能制造光学纳米生物传感界面的方法，（2）通过核酸扩增方法的生物传感机制，（3）最新策略和未来展望。
