Chen Haoxiang, Zhou Xi, Wang Miao, Ren Lei
The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
State Key Lab of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China.
J Funct Biomater. 2023 Feb 10;14(2):97. doi: 10.3390/jfb14020097.
Rapid, accurate, and portable on-site detection is critical in the face of public health emergencies. Infectious disease control and public health emergency policymaking can both be aided by effective and trustworthy point of care tests (POCT). A very promising POCT method appears to be the clustered regularly interspaced short palindromic repeats and associated protein (CRISPR/Cas)-based molecular diagnosis. For on-site detection, CRISPR/Cas-based detection can be combined with multiple signal sensing methods and integrated into smart devices. In this review, sensing methods for CRISPR/Cas-based diagnostics are introduced and the advanced strategies and recent advances in CRISPR/Cas-based POCT are reviewed. Finally, the future perspectives of CRISPR and POCT are summarized and prospected.
在突发公共卫生事件面前，快速、准确且便携的现场检测至关重要。有效的即时检测（POCT）有助于传染病防控和突发公共卫生事件的政策制定。基于成簇规律间隔短回文重复序列及其相关蛋白（CRISPR/Cas）的分子诊断似乎是一种非常有前景的POCT方法。对于现场检测，基于CRISPR/Cas的检测可与多种信号传感方法相结合，并集成到智能设备中。本文综述了基于CRISPR/Cas诊断的传感方法，并对基于CRISPR/Cas的POCT的先进策略和最新进展进行了回顾。最后，总结并展望了CRISPR和POCT的未来前景。
