School of Life Sciences, BS Abdur Rahman University, Vandalur, Chennai, Tamil Nadu 600048, India.
Future Microbiol. 2018 Mar;13:297-312. doi: 10.2217/fmb-2017-0132. Epub 2018 Feb 15.
To understand the effects of gallic acid (GA) on ampicillin (Amp) sensitive or resistant strain of Pseudomonas sp. and also in modulating the corresponding biofilms.
The cell viability was determined by broth dilution, dry weight and CFU assays. Biofilm formation was measured by crystal violet assay while oxygen consumption rate was measured to verify the metabolic status of the cells. The membrane damage and drug efflux/accumulation were studied by fluorimetric assays.
GA transformed the Amp resistant cells, both planktonic and biofilms, into highly sensitive one by inducing membrane damage and enhancing accumulation of drug, whereas the Amp sensitive cells gained resistance against Amp.
Use of GA as an antimicrobial compound should be analyzed more critically depending on the drug dosages, drug sensitivity as well as types of bacterial strains being studied.
了解没食子酸（GA）对铜绿假单胞菌敏感或耐药菌株的影响，以及对相应生物膜的调节作用。
通过肉汤稀释法、干重法和 CFU 测定法测定细胞活力。通过结晶紫法测定生物膜形成，通过耗氧率测定来验证细胞的代谢状态。通过荧光测定法研究细胞膜损伤和药物外排/积累。
GA 通过诱导细胞膜损伤和增强药物积累，将 Amp 耐药的浮游细胞和生物膜细胞转化为高度敏感的细胞，而 Amp 敏感的细胞则对 Amp 产生了耐药性。
根据药物剂量、药物敏感性以及所研究的细菌菌株类型，应更仔细地分析 GA 作为抗菌化合物的使用。
