Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin, Xinjiang Production & Construction Group, Tarim University, Alar, China.
Xinjiang Production & Construction Group Key Laboratory of Agricultural Products Processing in Xinjiang South, College of Life Sciences, Tarim University, Alar, China.
Curr Microbiol. 2020 Jul;77(7):1167-1173. doi: 10.1007/s00284-019-01847-w. Epub 2020 Feb 18.
Staphylococcus epidermidis is one of the main causes of medical device-related infections and bovine mastitis owing to its biofilm-forming abilities. Oxytropis glabra DC. is one of the most widespread Fabaceae species and used as a Chinese herbal formulation in Western China. Our research investigated the effects of O. glabra on the biofilm formation of S. epidermidis and the possible inhibiting mechanism. The biofilm-forming reference strain, S. epidermidis SE-1 (ATCC 35,984), was employed as a model and semi-quantitative biofilm assay was performed to evaluate the antibiofilm activity of O. glabra. The exopolysaccharides (EPS) production and expression of ica operon were studied to explore the possible antibiofilm mechanism using thin-layer chromatography and quantitative real-time PCR assay, respectively. The results obtained indicated that O. glabra decoction at 7.5 mg mL significantly inhibited biofilm formation by about 95% without affecting cell growth of S. epidermidis. Two hydrolysis productions of EPS were significantly decreased by 64% and 54% with the addition of 7.5 mg mLO. glabra and the expression of icaR was significantly up-regulated 2.2-times, whereas icaB was significantly down-regulated more than 50% by 7.5 mg mLO. glabra. These findings suggest a potential application for O. glabra as a promising candidate for the exploration of new drugs against S. epidermidis biofilm-associated infections.
表皮葡萄球菌因其形成生物膜的能力，是医疗器械相关感染和奶牛乳腺炎的主要原因之一。甘青青兰是最广泛的豆科物种之一，在中国西部被用作中草药配方。我们的研究调查了甘青青兰对表皮葡萄球菌生物膜形成的影响及其可能的抑制机制。使用表皮葡萄球菌 SE-1（ATCC 35,984）作为模式参考菌株进行半定量生物膜测定，评估甘青青兰的抗生物膜活性。通过薄层层析法和定量实时 PCR 分析分别研究了胞外多糖 (EPS) 的产生和 ica 操纵子的表达，以探讨可能的抗生物膜机制。结果表明，甘青青兰汤剂在 7.5 mg mL 时可显著抑制生物膜形成，抑制率约为 95%，而不影响表皮葡萄球菌的细胞生长。添加 7.5 mg mLO. glabra 后，EPS 的两种水解产物分别减少了 64%和 54%，而 icaR 的表达则显著上调了 2.2 倍，而 icaB 的表达则显著下调了 50%以上。这些发现表明，甘青青兰可能具有应用潜力，可以作为探索抗表皮葡萄球菌生物膜相关感染新药的候选药物。
