Rimal Binayak, Chang James D, Liu Chengyin, Kim Haley, Aderotoye Oluwatobi, Zechmann Bernd, Kim Sung Joon
Institute of Biomedical Studies, Baylor University, Waco, Texas 76798, United States.
Department of Chemistry and Biochemistry, Baylor University, Waco, Texas 76798, United States.
ACS Omega. 2024 Aug 27;9(36):37610-37620. doi: 10.1021/acsomega.4c01168. eCollection 2024 Sep 10.
Understanding the dynamics of biofilm formation and its elemental composition is crucial for developing effective strategies against biofilm-associated infections. In this study, we employed scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) to investigate the morphological changes and elemental compositions of biofilms. SEM images revealed distinct stages of biofilm development, from initial aggregation to the formation of mature and aged biofilms. EDS analysis consistently showed elevated levels of sodium (Na), oxygen (O), and phosphorus (P) in the biofilm matrix, indicating its high negative charge and the presence of anionic biopolymers. The incorporation of extracellular DNA (eDNA) into the biofilm matrix, leading to significant retention of sodium ions, underscored the importance of electrostatic interactions in biofilm formation and stability. Our findings highlight the potential of EDS analysis in quantifying elemental compositions and elucidating the role of anionic biopolymers in biofilm development.
了解生物膜形成的动力学及其元素组成对于制定有效的抗生物膜相关感染策略至关重要。在本研究中，我们使用扫描电子显微镜（SEM）和能量色散X射线光谱（EDS）来研究生物膜的形态变化和元素组成。SEM图像揭示了生物膜发育的不同阶段，从初始聚集到成熟和老化生物膜的形成。EDS分析一致显示生物膜基质中钠（Na）、氧（O）和磷（P）的含量升高，表明其高负电荷和阴离子生物聚合物的存在。细胞外DNA（eDNA）掺入生物膜基质导致钠离子的显著保留，强调了静电相互作用在生物膜形成和稳定性中的重要性。我们的研究结果突出了EDS分析在量化元素组成和阐明阴离子生物聚合物在生物膜发育中的作用方面的潜力。
