Zheng Jiawen, Li Zhuosi, Manabe Yuki, Kim Minji, Goto Tsuyoshi, Kawada Teruo, Sugawara Tatsuya
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 611-0011, Japan.
Lipids. 2018 Jan;53(1):41-52. doi: 10.1002/lipd.12002. Epub 2018 Feb 15.
Nonalcoholic fatty liver disease (NAFLD) has shown an increasing morbidity in recent years. Here, we demonstrated that siphonaxanthin (SPX), a rare marine carotenoid, exhibits a strong inhibitory effect on aggravated hepatic lipogenesis in vitro and would be a promising candidate in the prevention and alleviation of NAFLD in the future. In this study, we conducted a preliminary assessment of the effect of SPX on hepatic lipogenesis by using the HepG2 cell line, derived from human liver cancer, as a model of the liver. SPX significantly suppressed the excess accumulation of triacylglycerol induced by liver X receptor α (LXRα) agonist by downregulating a nuclear transcription factor named sterol regulatory element-binding protein-1c and a set of related genes. Moreover, fatty acid translocase (CD36) and fatty acid-binding protein-1, which regulates fatty acid uptake, also exhibited significant decrease in transcriptional levels. Furthermore, we found that SPX blocked LXRα activation and would be a promising candidate for antagonist of LXRα.
近年来，非酒精性脂肪性肝病（NAFLD）的发病率呈上升趋势。在此，我们证明了虹彩叶黄素（SPX），一种罕见的海洋类胡萝卜素，在体外对肝脏脂肪生成加剧具有强烈的抑制作用，并且在未来预防和缓解NAFLD方面将是一个有前景的候选物。在本研究中，我们以源自人类肝癌的HepG2细胞系作为肝脏模型，对SPX对肝脏脂肪生成的作用进行了初步评估。SPX通过下调一种名为固醇调节元件结合蛋白-1c的核转录因子及一组相关基因，显著抑制了肝脏X受体α（LXRα）激动剂诱导的三酰甘油过量积累。此外，调节脂肪酸摄取的脂肪酸转运蛋白（CD36）和脂肪酸结合蛋白-1的转录水平也显著降低。此外，我们发现SPX可阻断LXRα的激活，并且有望成为LXRα的拮抗剂。
