a Department of Otorhinolaryngology-Head and Neck Surgery , College of Medicine, Seoul National University , Korea.
b Nano-Bio Regenerative Medical Institute , Collage of Medicine, Hallym University , Chuncheon , Korea.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):885-895. doi: 10.1080/21691401.2018.1439039. Epub 2018 Feb 15.
Current treatments of oesophageal diseases, such as carcinoma, congenital abnormality or trauma, require surgical intervention and oesophageal reconstruction with the stomach, jejunum or colon. However, serious side effects are possible with each treatment option. Despite tissue engineering promising to be an effective regenerative strategy, no functional solution currently exists for oesophageal reconstruction. Here, we developed an omentum-cultured oesophageal scaffold reinforced by a 3D-printed ring. The nano-structured scaffolds were wrapped into the omentum of rats and orthotopically transplanted for the repair of circumferential oesophageal defects two weeks later. The artificial oesophagus exhibited complete healing of the surgically created circumferential defects by the second week. The integration of the omentum-cultured oesophageal scaffold and the regenerative tissue remained intact. Macroscopically, there was no evidence of a fistula, perforation, abscess formation or surrounding soft-tissue necrosis. The omentum-cultured nano-structure scaffold reinforced by a 3D-printed ring is a more practical model with better vascularization for artificial neo-oesophagus reconstruction in a rat model.
目前，食管疾病（如癌、先天异常或创伤）的治疗需要手术干预，并使用胃、空肠或结肠进行食管重建。然而，每种治疗方法都可能产生严重的副作用。尽管组织工程有望成为一种有效的再生策略，但目前对于食管重建还没有功能性的解决方案。在这里，我们开发了一种由 3D 打印环增强的带蒂网膜培养的食管支架。纳米结构支架被包裹在大鼠的网膜中，两周后进行原位移植以修复环形食管缺损。人工食管在第二周完全愈合了手术造成的环形缺损。带蒂网膜培养的食管支架和再生组织的整合保持完整。宏观上，没有瘘管、穿孔、脓肿形成或周围软组织坏死的证据。3D 打印环增强的带蒂网膜培养纳米结构支架是一种更实用的模型，可为大鼠模型中的人工新食管重建提供更好的血管化。
