Jiménez-López Julia, Ortega-Barrales Pilar, Ruiz-Medina Antonio
a Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences , University of Jaén , Jaén , Spain.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 May;35(5):941-949. doi: 10.1080/19440049.2018.1440640. Epub 2018 Feb 22.
One of the most used agrochemicals in agricultural production, nitenpyram (NTP), has been determined by using a flow-through optosensing device based on Photochemically Induced Fluorescence detection. The combination of both methodologies allows, on one hand, a quick on-line photodegradation of NTP and, on the other hand, the preconcentration, quantification and desorption of the fluorescent photoproduct generated when retained on Sephadex QAE-A25 as solid support, which was monitored at 295 and 362 nm for excitation and emission, respectively. The proposed analytical method presents a detection limit of 500 pg mL by using Multicommutated Flow Injection Analysis. Recovery experiments were carried out in different kinds of cruciferous vegetables at or below the MRL established in Japan, demonstrating that this method combines advantages of simplicity, high sensibility and high selectivity, fulfilling the requirements for its application in quality control. Results obtained in the analysis of real samples were in good agreement with those provided by a reference HPLC method.
烯啶虫胺（NTP）是农业生产中最常用的农用化学品之一，已通过基于光化学诱导荧光检测的流通式光传感装置进行了测定。这两种方法的结合，一方面可以使NTP快速在线光降解，另一方面可以对保留在作为固体支持物的葡聚糖QAE - A25上时产生的荧光光产物进行预浓缩、定量和解吸，分别在295nm和362nm处监测激发和发射。所提出的分析方法通过多通道流动注射分析给出的检测限为500 pg/mL。在日本规定的最大残留限量（MRL）及以下水平，对不同种类的十字花科蔬菜进行了回收率实验，结果表明该方法具有简单、高灵敏度和高选择性的优点，满足其在质量控制中的应用要求。实际样品分析结果与参考高效液相色谱法提供的结果高度一致。
