Department of Chemistry and State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China.
Dalton Trans. 2020 May 7;49(17):5445-5453. doi: 10.1039/d0dt00031k. Epub 2020 Apr 8.
A completely water-soluble, high quantum yield blue-fluorescent benzimidazole derivative (AQ), containing a rigid benzimidazole-thiophene structure, was synthesized. Among 21 metal ions, the fluorescence of AQ was selectively turned off by Cu to form an AQ-Cu ensemble. Thereafter, the fluorescence of the AQ-Cu ensemble was turned on by sulfide (S) with high selectivity and sensitivity in pure water solution. In comparison with AQ-Ag and AQ-Hg ensembles, AQ-Cu was the only ensemble that was capable of detecting a sulfide anion. Also, the fluorescence intensity of AQ was linearly proportional to the concentration of Cu and S. Both Cu and S were detected within a minute in vitro. Moreover, AQ worked best in the pH range of 5-10 and had a limit of detection of 50 nM and 354 nM for Cu and S respectively. It was employed for the detection of sulfide in human lung cancer A549 cells with low cytotoxicity.
一种完全水溶性、高量子产率的蓝色荧光苯并咪唑衍生物（AQ）被合成出来，它含有刚性的苯并咪唑-噻吩结构。在 21 种金属离子中，AQ 的荧光被 Cu 选择性地关闭，形成 AQ-Cu 配合物。此后，AQ-Cu 配合物在纯水溶液中通过硫（S）以高选择性和灵敏度被重新打开。与 AQ-Ag 和 AQ-Hg 配合物相比，AQ-Cu 是唯一能够检测到硫阴离子的配合物。此外，AQ 的荧光强度与 Cu 和 S 的浓度呈线性关系。在体外，Cu 和 S 都可以在一分钟内被检测到。此外，AQ 在 pH 值为 5-10 的范围内效果最佳，对 Cu 和 S 的检测限分别为 50 nM 和 354 nM。它被用于检测人肺癌 A549 细胞中的硫，具有低细胞毒性。
