Aparna T K, Sivasubramanian R
Nanosensor Laboratory, PSG Institute of Advanced Studies, Coimbatore 641004, India.
J Nanosci Nanotechnol. 2018 Feb 1;18(2):789-797. doi: 10.1166/jnn.2018.13968.
Three dimensional flower-like NiO-thermally reduced Graphene Oxide (trGO) nanocomposite was prepared by a simple hydrothermal method. The morphology and structure of the nanocomposite was characterized using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Energy Dispersive X-ray (EDX), X-ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The electrocatalytic oxidation of Dopamine, Ascorbic Acid (AA) and Uric Acid (UA) was investigated using Cyclic Voltammetry (CV). The modified electrode NiO-trGO/GCE showed good electrocatalytic activity compared to bare GCE. The detection of dopamine was carried out using Differential Pulse Voltammetry (DPV) wherefrom the Limit of Detection (LOD) and linear range was estimated to be 50 nM and 10 μM to 500 μM respectively. The selective detection of dopamine in presence of UA and AA was carried out. The NiO-trGO/GCE showed excellent selectivity for the detection of dopamine in presence of UA. However AA was found to interfere in the determination of dopamine. Also, the validity of the present sensor was examined in human biological samples.
采用简单的水热法制备了三维花状氧化镍-热还原氧化石墨烯（trGO）纳米复合材料。利用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散X射线（EDX）、X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱对该纳米复合材料的形貌和结构进行了表征。采用循环伏安法（CV）研究了多巴胺、抗坏血酸（AA）和尿酸（UA）的电催化氧化。与裸玻碳电极（GCE）相比，修饰电极NiO-trGO/GCE表现出良好的电催化活性。使用差分脉冲伏安法（DPV）对多巴胺进行检测，据此估计检测限（LOD）和线性范围分别为50 nM以及10 μM至500 μM。在存在尿酸和抗坏血酸的情况下对多巴胺进行了选择性检测。NiO-trGO/GCE在存在尿酸的情况下对多巴胺的检测表现出优异的选择性。然而，发现抗坏血酸会干扰多巴胺的测定。此外，还在人体生物样品中检验了该传感器的有效性。
