Biocidal Activity of Metal Nanoparticles Synthesized by against Multidrug-Resistant Bacteria and Mycotoxigenic Fungi.

Antibiotic resistance by pathogenic bacteria and fungi is one of the most serious global public health problems in the 21 century, directly affecting human health and lifestyle. and with strong resistance to the common antibiotics have been isolated from Intensive Care Unit patients at Zagazig Hospital. Thus, in this study we assessed the biocidal activity of nanoparticles of silver, copper and zinc synthesized by KJ 623702 against these multidrug resistant-bacteria. The synthesized Metal Nano-particles (MNPs) were characterized by UV-Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and Zeta potential. The Fourier transform infrared spectroscopy (FTIR) result showed the presence of different functional groups such as carboxyl, amino and thiol, ester and peptide bonds in addition to glycosidic bonds that might stabilize the dispersity of MNPs from aggregation. The antimicrobial potential of MNPs by against the multidrug-resistant (MDR) and in addition to the mycotoxigenic , and was investigated, based on the visual growth by diameter of inhibition zone. Among the synthesized MNPs, the spherical AgNPs (13.70 nm) displayed significant effect against (Zone of Inhibition 22.4 mm and Minimum Inhibitory Concentration 21.33 µg/ml), while ZINC oxide Nano-Particles were the most effective against (ZOI, 18.5 mm and MIC 24.7 µg/ml). Transmission Electron Microscope micrographs of AgNP-treated showed cracks and pits in the cell wall, with internalization of NPs. Production of pyocyanin pigment was significantly inhibited by AgNPs in a concentration-dependent manner, and at 5-20 µg of AgNPs/ml, the pigment production was reduced by about 15-100%, respectively.