细菌在亚抑制水平的林可酰胺类或壮观霉素相关抗生素中生长后对血清溶解或吞噬作用的敏感性。

Susceptibility of bacteria to serum lysis or phagocytosis following growth in subinhibitory levels of lincosaminide or spectinomycin related antibiotics.

作者信息

Cialdella J I, Vavra J J, Marshall V P

出版信息

J Antibiot (Tokyo). 1986 Jul;39(7):978-84. doi: 10.7164/antibiotics.39.978.

DOI:10.7164/antibiotics.39.978

Abstract

The effects of subinhibitory concentrations of antibiotics on polymorphonuclear leukocyte (PML) and serum killing of Staphylococcus aureus 502A (UC 9116) and Escherichia coli UC 9451 were studied. Exposure of these bacteria to subinhibitory levels of certain lincosaminides, spectinomycin, or 6'-n-propylspectinomycin altered their susceptibility to these host defense mechanisms, while exposure to gentamicin had no effect. However, each organism responded differently to treatment with the antibiotics. S. aureus pretreated during log phase growth with subinhibitory concentrations of clindamycin, lincomycin, or pirlimycin was more susceptible to killing by PMLs than untreated bacteria. No effect on phagocytic killing was found when S. aureus was pretreated with spectinomycin, 6'-n-propylspectinomycin, or gentamicin. The S. aureus remained resistant to serum lysis despite antibiotic treatment. In contrast, spectinomycin and 6'-n-propylspectinomycin as well as clindamycin dramatically increased the susceptibility of E. coli to serum lysis (greater than 99% destroyed). Moderate killing of E. coli by PMLs was also found.

摘要

研究了亚抑菌浓度的抗生素对多形核白细胞（PML）以及对金黄色葡萄球菌502A（UC 9116）和大肠杆菌UC 9451的血清杀菌作用的影响。将这些细菌暴露于某些林可酰胺类、壮观霉素或6'-正丙基壮观霉素的亚抑菌水平会改变它们对这些宿主防御机制的敏感性，而暴露于庆大霉素则没有影响。然而，每种生物体对抗生素治疗的反应不同。在对数生长期用亚抑菌浓度的克林霉素、林可霉素或匹利霉素预处理的金黄色葡萄球菌比未处理的细菌更易被PMLs杀死。当金黄色葡萄球菌用壮观霉素、6'-正丙基壮观霉素或庆大霉素预处理时，未发现对吞噬杀伤有影响。尽管进行了抗生素治疗，金黄色葡萄球菌仍对血清溶解有抗性。相比之下，壮观霉素、6'-正丙基壮观霉素以及克林霉素显著增加了大肠杆菌对血清溶解的敏感性（大于99%被破坏）。还发现PMLs对大肠杆菌有适度的杀伤作用。