Department of Biotechnology, Centre for Genetic studies, School of Biotechnology and Biological Sciences, West Bengal University of Technology, BF-142, Sector I, Salt Lake City, Kolkata, West Bengal 70064, India.
Department of Periodontia, North Bengal Dental College, Siliguri, West Bengal, 734012, India.
Biosci Rep. 2018 Jul 31;38(4). doi: 10.1042/BSR20171212. Print 2018 Aug 31.
Periodontitis is a very common inflammatory oral disease. Tumor necrosis factor-α (TNF-α) is a cytokine that has been involved with the gingival tissue destruction and remodeling occurrence. We investigated the association of single nucleotide polymorphisms (SNPs) in TNF-α gene promoter region with the susceptibility of aggressive and chronic periodontitis in the eastern Indian population. A total of 397 DNA samples from venous blood were isolated. 40 individuals were aggressive periodontitis patients, 157 were identified chronic periodontitis patients, and the remaining 200 were healthy individuals. Five SNPs of TNF-α at promoter region (rs361525, rs1800629, rs1799724, rs1800630, and rs1799964) were genotyped by PCR-sequencing in periodontitis patients and control subjects. rs1800629 (-308G/A) polymorphism was more frequent in both aggressive and chronic periodontitis patients compared with the control population, though the allele frequency was different only in aggressive periodontitis patients. On the other hand, both the genotypic and allelic variation of rs361525 (-238G/A) polymorphism were found significantly less frequently in aggressive and chronic periodontitis than in controls. The other polymorphisms like rs1799724 (-857C/T) and rs1799964 (-1031T/C) were significantly different between chronic periodontitis patients and control subjects. The findings suggest that the rs1800629 (-308G/A) polymorphism of TNF-α gene is associated with both aggressive and chronic periodontitis while rs1799724 (-857C/T) and rs1799964 (-1031T/C) polymorphisms of TNF-α gene is associated only with the increased susceptibility to chronic periodontitis.
牙周炎是一种非常常见的炎症性口腔疾病。肿瘤坏死因子-α（TNF-α）是一种细胞因子，与牙龈组织破坏和重塑的发生有关。我们研究了 TNF-α 基因启动子区域的单核苷酸多态性（SNPs）与侵袭性和慢性牙周炎易感性的关联在印度东部人群中。从静脉血中分离出 397 个 DNA 样本。40 人为侵袭性牙周炎患者，157 人为慢性牙周炎患者，其余 200 人为健康人。通过聚合酶链反应-测序对 TNF-α 基因启动子区域的 5 个 SNP（rs361525、rs1800629、rs1799724、rs1800630 和 rs1799964）进行基因分型。与对照组相比，rs1800629（-308G/A）多态性在侵袭性和慢性牙周炎患者中更为常见，尽管等位基因频率仅在侵袭性牙周炎患者中不同。另一方面，rs361525（-238G/A）多态性的基因型和等位基因变异在侵袭性和慢性牙周炎患者中均明显低于对照组。其他多态性，如 rs1799724（-857C/T）和 rs1799964（-1031T/C），在慢性牙周炎患者和对照组之间存在显著差异。这些发现表明，TNF-α 基因的 rs1800629（-308G/A）多态性与侵袭性和慢性牙周炎有关，而 TNF-α 基因的 rs1799724（-857C/T）和 rs1799964（-1031T/C）多态性仅与慢性牙周炎易感性增加有关。
