Lavu Vamsi, Venkatesan Vettriselvi, Venugopal Priyanka, Lakkakula Bhaskar Venkata Kameswara Subrahmanya, Paul Solomon Franklin Durairaj, Peria Kumarasamy, Rao Suresh Ranga
Department of Periodontics, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, Tamil Nadu, India.
Iran J Immunol. 2017 Mar;14(1):51-58.
Cytokines are suggested to play a role in periodontitis.
To determine and compare the levels of Interleukin-1 beta (IL-1β) and Tumor necrosis factor alpha (TNF-α) in gingival crevicular fluid (GCF) samples amongst healthy individuals and those with chronic periodontitis. Further to compare the GCF cytokine levels in three genotype classes defined by the respective gene polymorphisms.
The study was conducted on 41 chronic periodontitis patients and 40 healthy volunteers. IL-1β and TNF-α were quantified in GCF by cytometric bead array. DNA was extracted from peripheral blood samples and genotyping of IL1B +3954C/T (rs1143634) IL1B -511G/A (rs16944), TNFA -1031T/C (rs1799964) and TNFA -863C/A (rs1800630) polymorphisms were performed using Sanger sequencing and Taqman SNP genotyping assays methods.
Both IL-1β and TNF-α levels were significantly higher in chronic periodontitis group compared to the controls. IL-1β and TNF-α levels did not significantly differ in genotype classes of the respective polymorphism (IL1B -511G/A, TNFA -1031T/C and TNFA -863C/A). However, individuals with CT genotype of IL1B +3954C/T showed higher levels of IL-1β in the gingival crevicular fluid (ANOVA p<0.05).
The results of this study revealed the presence of higher levels of IL-1β and TNF-α in subjects with periodontitis and genetic control of IL-1β levels in our samples of Indians.
细胞因子被认为在牙周炎中起作用。
测定并比较健康个体和慢性牙周炎患者龈沟液（GCF）样本中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的水平。进一步比较由各自基因多态性定义的三种基因型类别的GCF细胞因子水平。
对41例慢性牙周炎患者和40名健康志愿者进行研究。通过细胞计数珠阵列对GCF中的IL-1β和TNF-α进行定量。从外周血样本中提取DNA，并使用桑格测序和Taqman SNP基因分型检测方法对IL1B +3954C/T（rs1143634）、IL1B -511G/A（rs16944）、TNFA -1031T/C（rs1799964）和TNFA -863C/A（rs1800630）多态性进行基因分型。
与对照组相比，慢性牙周炎组的IL-1β和TNF-α水平均显著更高。各自多态性（IL1B -511G/A、TNFA -1031T/C和TNFA -863C/A）的基因型类别中，IL-1β和TNF-α水平无显著差异。然而，IL1B +3954C/T的CT基因型个体龈沟液中的IL-1β水平更高（方差分析p<0.05）。
本研究结果显示牙周炎患者中IL-1β和TNF-α水平较高，且在我们的印度样本中IL-1β水平受基因控制。
